Catálogo de publicaciones - revistas

<jats:p>In the mitochondrial outer membrane, α-helical transmembrane proteins play critical roles in cytoplasmic-mitochondrial communication. Using genome-wide CRISPR screens, we identified mitochondrial carrier homolog 2 (MTCH2), and its paralog MTCH1, and showed that it is required for insertion of biophysically diverse tail-anchored (TA), signal-anchored, and multipass proteins, but not outer membrane β-barrel proteins. Purified MTCH2 was sufficient to mediate insertion into reconstituted proteoliposomes. Functional and mutational studies suggested that MTCH2 has evolved from a solute carrier transporter. MTCH2 uses membrane-embedded hydrophilic residues to function as a gatekeeper for the outer membrane, controlling mislocalization of TAs into the endoplasmic reticulum and modulating the sensitivity of leukemia cells to apoptosis. Our identification of MTCH2 as an insertase provides a mechanistic explanation for the diverse phenotypes and disease states associated with MTCH2 dysfunction.</jats:p>

<jats:p> Spermidine (SPD) delays age-related pathologies in various organisms. SPD supplementation overcame the impaired immunotherapy against tumors in aged mice by increasing mitochondrial function and activating CD8 <jats:sup>+</jats:sup> T cells. Treatment of naïve CD8 <jats:sup>+</jats:sup> T cells with SPD acutely enhanced fatty acid oxidation. SPD conjugated to beads bound to the mitochondrial trifunctional protein (MTP). In the MTP complex, synthesized and purified from <jats:italic>Escherichia coli</jats:italic> , SPD bound to the α and β subunits of MTP with strong affinity and allosterically enhanced their enzymatic activities. T cell–specific deletion of the MTP α subunit abolished enhancement of programmed cell death protein 1 (PD-1) blockade immunotherapy by SPD, indicating that MTP is required for SPD-dependent T cell activation. </jats:p>

<jats:p>Merck unearths a frozen batch of an experimental vaccine it made years ago</jats:p>

<jats:p>How exploding stars forge elements could be harder to trace</jats:p>

<jats:p> Instead, <jats:italic>eLife</jats:italic> will offer to peer review selected submissions for $2000 fee, then make paper and critiques free to read </jats:p>

<jats:p>“Swientists” hunt for influenza virus at hog shows, hoping to cut off the next pandemic at the pen</jats:p>

<jats:p>The skull shapes of mammals diversified more rapidly early in their history</jats:p>

<jats:p>The human genome contains a domesticated viral envelope gene with antiviral activity</jats:p>