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In this study, the double recombinant vaccinia viruses were generated those express exogenous proteins: human granulocyte-macrophage colony-stimulating factor (GM-CSF) and the antitumor protein lactaptin in secreted and nonsecreted forms. We observed that recombinant VV-GMCSF-Lact with nonsecreted lactaptin exerted stronger cytotoxic activity than others in MDA-MB-231, BT-549 and BT-20 breast cancer cells with calculated CD of 0.005; 0.004 и 0.00083 PFU/cell correspondently. Strain VV-GMCSF-Lact also exhibited highest lytic activity in lung cancer cells H1299 and epidermoid carcinoma cells A-431. Normal MCF10A cells and diploid embryonic lung human cells LECH-240 were resistant to all recombinant vaccinia viruses. Strain VV-GMCSF-Lact showed the highest index of tumor selectivity in pairs normal/cancer cells: MCF10A/MDA-MB-231 (>2000) and LECH-240/H1299 (190). By flow cytometry, we demonstrated that all recombinants induced apoptosis in treated cancer cells but the rate of annexin V-positive cells was higher after treatment with VV-GMCSF-Lact than others. Thus nonsecreted lactaptin expression increased the toxicity of recombinant virus to cancer cells in the best way. It is likely that lactaptin expression inside the treated cells (without secretion outside) intensifies apoptosis and as a consequence promotes the progression of apoptotic cells to secondary necrotic cells. These results demonstrate that recombinant VV-GMCSF-Lact has good oncolytic potential and stimulate further investigation of its anticancer activity in human tumor models in vivo and to use it in the development of anticancer therapeutic agents.

We performed the genome-wide association study of estimated breeding values for milk production traits in Russian Holstein and black-and-white cattle population. The join dairy cows’ population of Moscow and Leningrad regions was used to create a common reference group of animals to obtain the genomic breeding values. We identified breeding and genetic parameters for milk yield for 305 days of lactation, milk fat and protein content, milk fat and protein yield. We found several high-significant conservative mutations associated with milk fat content (e.g., ,  = 6.8 × 10), as well as a 1.5 Mb locus on BTA14. Our results will be used to develop a genomic evaluation programs, aimed to improve economically important traits in dairy cattle in Russia.

Screening for new antibacterial compounds is an urgent need of medicinal chemistry. Understanding new antibiotics mechanism of action is needed for progression in the drug development pipeline. In the frame of the project supported by the Ministry of Science, we developed a reporter system which allows an express, cost-effective and high-throughput screening for simultaneous detection of antibacterial activity, protein synthesis inhibition and induction of DNA damage SOS response. Automation of the screening process developed in the frame of this project allowed to screen up to 17,856 compound chemical library, supplied by the industrial partner of the project, Research Institute of Chemical Diversity. Among the tested compounds, DNA damaging agents appeared almost sixfold more frequently than those that inhibited protein synthesis. Several new families of antibacterial compounds were found among the tested set.

Analysis of the shapes of voltage–frequency curves depending on the type of object detached from the surface of the quartz crystal microbalance (QCM) is carried out. It is demonstrated that the shape of the curve depends not only on the size and shape of bio-object but also on the properties of the particle. For example, a detachment of hepatitis B virus is accompanied by the fragmentation of the bio-object, and signal shape is typical for this case. In addition to a voltage value which determines the bonding force, the signal shape is also characteristic for identification of bio-object.

The technology of processing sludge includes electromagnetic activation of raw materials, catalytic cracking, hydrocracking, and oxidative desulfurization. Preliminary activation reduces electromagnetic temperature catalytic processes at 60–80 ℃, increasing the yield of light products, reduce sulfur content in liquid products at 13–19% by weight, coke formation, and reduces gassing.

In this paper, we describe the developed methods and technologies, as well as the created research prototype of a ground-space regional monitoring system that was used for comprehensive experimental research of anthropogenic impact on Russian Black sea coastal water areas. Changes in significant water environment parameters (generation of additional spectral components of surface waves; changes in marine surface roughness affecting normalized radar cross-section; turbidity field anomalies affecting spectral brightness variations in various bands of electromagnetic spectrum, etc.) registered in satellite imagery of water areas under anthropogenic impact were revealed. It has been established that these effects were predominantly caused by deep wastewater discharges. Zones of anthropogenic pollution propagations, as well as pipe breakages, have been revealed. The validation of the obtained satellite imagery processing results has been conducted based on sea truth data carried out using buoys, as well as from boats and hydrophysical platform.

The future development of mineral raw material base in alumina’s production is vary widely with resources development of low-grade alumina stock. It is actual for countries and regions with limit or stock out of traditional bauxite raw. Significant opportunities are presents the natural aluminosilicate as part of urtite, rischorrit, ijolite and the others alkali rocks and also the dump waste products of mine-mill consist of the alumosilicates incorporated overburden rocks and stocker’s middlings. At the same time essential to the efficient use such materials is considerations of their chemical and mineral composition, mole ratio in calciferous- aluminosilicate dry mix, sintering temperature and other parameters that determine the recovery of valuable raw material components. The future development of raw material base for the production of alumina in the central part of Siberia is strongly associated with the development Goryachegorsk deposits of nepheline ores.Experimental results is allow to clarify chemical and mineralogical composition of sample of nepheline concentrate’ obtained by Goryachegorsk field’s ore beneficiation and to determine the optimal conditions for its processing with the extraction in an alkaline aluminate solution alumina and alkaline components. The obtained results establish the possibility of achieving valuable components extraction rates of more than 90%, which exceeds or matches the existing level for the same raw materials, and combined with the implementation of other resource-saving solutions can rely on high efficiency of the process.

During cleaning of high-ash coal mainly “wet” processes are used which require 5–10 tonnes water consumption per 1 tonne of coal. Arrangement of recycling water supply reduces demand in “fresh” water, but transportation of huge volumes of water slurry requires high-energy consumption. Dry cleaning of low-rank coal which has not been exposed to preliminary preparation is inefficient. It was suggested that to provide dry cleaning of high-ash coal it would be reasonable to expose it to chemical heat treatment first, and then to direct the treated coal mass for physical and mechanical cleaning to get the low-ash high-caloric product. It has been determined that in black coal exposed to medium temperature pyrolysis, as well as in brown coal, improvement of incombustible mineral fraction liberation is observed that facilitates further beneficiation with the use of a combination of high-intensity magnetic separation and triboelectrostatic separation. It has been determined that cleaned semicoke substantially exceeds both initial and cleaned coal by its qualities as a solid fuel, and tailings of semicoke dry cleaning can be utilised.

The paper presents the results of studies aimed at obtaining experimental data for development of technological solutions to the production of marketable petrochemicals and petroleum products from heavy oil wastes using the process of hydroconversion and at designing engineering solutions in the area of preliminary treatment of heavy oil wastes for their further processing into marketable petrochemicals and petroleum products. The experiments have been conducted using a bench for heavy oil waste processing which combines pretreatment of oil wastes and subsequent hydroconversion processing in the presence of highly efficient ultrafine catalysts. Optimum conditions of oil sludge heavy residue hydroconversion (pressure, 7 MPa; temperature, 435 ℃; feedstock space velocity, 1 h; hydrogen: feedstock, 1000 nL/L; catalyst (Mo) content, 0.05 wt%; HO, 2 wt% (based on the feedstock) make it possible to achieve conversion of the 520 ℃+ fraction of feedstock of up to 67 wt% (per pass) or 90 wt% (based on the recycle stock).

In the paper presented are the results of laboratory studies on roily oils purification from mechanical impurities and petroleum products using methods and devices designed at the JSC “SSC RF—IPPE.” These studies were carried out within the framework of the Agreement on granting. These studies have demonstrated the effectiveness of nanostructured membranes for removal of mechanical impurities from water-oil emulsions and jet-film generator of air bubbles for floatation purification of emulsions from petroleum products. Nanostructured membranes are capable of completely removing mechanical impurities over 0.5 μm in size from model roily oils solutions resulting in neither impurities biofouling, nor pores plugging. Mechanical impurities are accumulated on the surface of the nanostructured membrane, and upon reaching their max permissible amount, regeneration of the membrane is carried out. As a result of regeneration, filtering element capacity is recovered up to 95–97% of its initial value. The possibility of production of finely divided air bubbles (less than 100 μm in size) by the jet-film generator was demonstrated and their high concentration in water was reached. Studies were carried out on the possibility of transport of finely divided air bubbles in the low speed (less than 2 m/min) water flow. The effectiveness of floatation purification method was tested under laboratory conditions by increasing amount of the air bubbles and decreasing their size. Taking into account the roily oils properties, the proposed methods of their purification seem quite promising.