Catálogo de publicaciones - revistas
Nature Biotechnology
Resumen/Descripción – provisto por la editorial en inglés
Nature Biotechnology is a monthly journal covering the science and business of biotechnology. It publishes new concepts in technology/methodology of relevance to the biological, biomedical, agricultural and environmental sciences as well as covers the commercial, political, ethical, legal, and societal aspects of this research. The first function is fulfilled by the peer-reviewed research section, the second by the expository efforts in the front of the journal. We provide researchers with news about business; we provide the business community with news about research developments.Palabras clave – provistas por la editorial
No disponibles.
Disponibilidad
| Institución detectada | Período | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No detectada | desde jul. 2012 / hasta dic. 2023 | Nature.com |
Información
Tipo de recurso:
revistas
ISSN impreso
1087-0156
ISSN electrónico
1546-1696
Editor responsable
Springer Nature
País de edición
Reino Unido
Fecha de publicación
1996-
Cobertura temática
Tabla de contenidos
Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo
Meirui An
; Aditya Raguram; Samuel W. Du; Samagya Banskota; Jessie R. Davis; Gregory A. Newby; Paul Z. Chen; Krzysztof Palczewski; David R. Liu
<jats:title>Abstract</jats:title><jats:p>Prime editing enables precise installation of genomic substitutions, insertions and deletions in living systems. Efficient in vitro and in vivo delivery of prime editing components, however, remains a challenge. Here we report prime editor engineered virus-like particles (PE-eVLPs) that deliver prime editor proteins, prime editing guide RNAs and nicking single guide RNAs as transient ribonucleoprotein complexes. We systematically engineered v3 and v3b PE-eVLPs with 65- to 170-fold higher editing efficiency in human cells compared to a PE-eVLP construct based on our previously reported base editor eVLP architecture. In two mouse models of genetic blindness, single injections of v3 PE-eVLPs resulted in therapeutically relevant levels of prime editing in the retina, protein expression restoration and partial visual function rescue. Optimized PE-eVLPs support transient in vivo delivery of prime editor ribonucleoproteins, enhancing the potential safety of prime editing by reducing off-target editing and obviating the possibility of oncogenic transgene integration.</jats:p>
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Tearing up the traditional biotech playbook
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
It takes two to think
Itai Yanai; Martin J. Lercher
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
A palmitoyl transferase chemical–genetic system to map ZDHHC-specific S-acylation
Cory A. Ocasio; Marc P. Baggelaar; James Sipthorp; Ana Losada de la Lastra; Manuel Tavares; Jana Volarić; Christelle Soudy; Elisabeth M. Storck; Jack W. Houghton; Susana A. Palma-Duran; James I. MacRae; Goran Tomić; Lotte Carr; Julian Downward; Ulrike S. Eggert; Edward W. Tate
<jats:title>Abstract</jats:title><jats:p>The 23 human zinc finger Asp-His-His-Cys motif-containing (ZDHHC) <jats:italic>S</jats:italic>-acyltransferases catalyze long-chain <jats:italic>S</jats:italic>-acylation at cysteine residues across an extensive network of hundreds of proteins important for normal physiology or dysregulated in disease. Here we present a technology to directly map the protein substrates of a specific ZDHHC at the whole-proteome level, in intact cells. Structure-guided engineering of paired ZDHHC ‘hole’ mutants and ‘bumped’ chemically tagged fatty acid probes enabled probe transfer to specific protein substrates with excellent selectivity over wild-type ZDHHCs. Chemical–genetic systems were exemplified for five human ZDHHCs (3, 7, 11, 15 and 20) and applied to generate de novo ZDHHC substrate profiles, identifying >300 substrates and <jats:italic>S</jats:italic>-acylation sites for new functionally diverse proteins across multiple cell lines. We expect that this platform will elucidate <jats:italic>S</jats:italic>-acylation biology for a wide range of models and organisms.</jats:p>
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Delivery of a sebum modulator by an engineered skin microbe in mice
Nastassia Knödlseder; María-José Fábrega; Javier Santos-Moreno; Joan Manils; Lorena Toloza; Maria Marín Vilar; Cristina Fernández; Katrina Broadbent; Julien Maruotti; Hélène Lemenager; Carlo Carolis; Christos C. Zouboulis; Concepció Soler; Rolf Lood; Holger Brüggemann; Marc Güell
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
High prevalence of acquired cancer-related mutations in 146 human pluripotent stem cell lines and their differentiated derivatives
Elyad Lezmi; Jonathan Jung; Nissim Benvenisty
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Prime editing using CRISPR-Cas12a and circular RNAs in human cells
Ronghong Liang; Zixin He; Kevin Tianmeng Zhao; Haocheng Zhu; Jiacheng Hu; Guanwen Liu; Qiang Gao; Meiyan Liu; Rui Zhang; Jin-Long Qiu; Caixia Gao
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Engineered serum markers for non-invasive monitoring of gene expression in the brain
Sangsin Lee; Shirin Nouraein; James J. Kwon; Zhimin Huang; Jessica A. Wojick; Boao Xia; Gregory Corder; Jerzy O. Szablowski
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Generating expression profiles of single living cells from Raman microscopy
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Monitoring gene expression in the brain with a blood test
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible