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Genetic Engineering: Principles and Methods

Jane K. Setlow (eds.)

Resumen/Descripción – provisto por la editorial

No disponible.

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Disponibilidad
Institución detectada Año de publicación Navegá Descargá Solicitá
No detectada 2006 SpringerLink

Información

Tipo de recurso:

libros

ISBN impreso

978-0-387-25855-3

ISBN electrónico

978-0-387-25856-0

Editor responsable

Springer Nature

País de edición

Reino Unido

Fecha de publicación

Información sobre derechos de publicación

© Springer-Verlag US 2006

Tabla de contenidos

Biogenesis of Iron-Sulfur Cluster Proteins in Plastids

Iron-sulfur (Fe-S) clusters are co-factors of proteins that perform a number of biological roles, including electron transfer, redox and non-redox catalysis, regulation of gene expression, and as sensors within all living organisms, prokaryotes and eukaryotes. These clusters are thought to be among the oldest structures found in biological cells. In chloroplasts, Fe-S clusters play a key role in photosynthetic electron transport as well as nitrogen and sulfur assimilation. The capacity of the Fe atom in Fe-S clusters to take up an electron reversibly provides the required electron carrier capacity in these pathways. Iron and sulfur limitation both affect plant primary production and growth. It has long been known that iron deficiency leads to defects in photosynthesis and bleaching in young leaves, phenomena that are closely linked to a defect in chloroplastic photosystem- I (PSI) accumulation, a major Fe-S containing protein complex in plants. Although the functional importance of Fe-S cluster proteins is evident and isolated chloroplasts have been shown to be able to synthesize their own Fe-S clusters, much is yet to be learned about the biosynthesis of Fe-S proteins in plastids. The recent discovery of a NifS-like protein in plastids has hinted to the existence of an assembly machinery related to bacterial Fe-S assembly systems. This chapter aims to summarize what we presently know about the assembly of Fe-S clusters in plants with an emphasis on green plastids.

Palabras clave: Sulfite Reductase; Sulfur Assimilation; Cysteine Desulfurase; Reconstitution Activity; Selenocysteine Lyase.

Pp. No disponible

Strategies for High-Throughput Gene Cloning and Expression

High-throughput approaches for gene cloning and expression require the development of new, nonstandard tools for use by molecular biologists and biochemists. We have developed and implemented a series of methods that enable the production of expression constructs in 96-well plate format. A screening process is described that facilitates the identification of bacterial clones expressing soluble protein. Application of the solubility screen then provides a plate map that identifies the location of wells containing clones producing soluble proteins. A series of semi-automated methods can then be applied for validation of solubility and production of freezer stocks for the protein production group. This process provides an 80% success rate for the identification of clones producing soluble protein and results in a significant decrease in the level of effort required for the labor-intensive components of validation and preparation of freezer stocks. This process is customized for large-scale structural genomics programs that rely on the production of large amounts of soluble proteins for crystallization trials.

Palabras clave: Soluble Protein; Assay Plate; Expression Clone; Ranking Data; Ligation Independent Cloning.

Pp. No disponible

Identification and Analysis of Micrornas

Shveta Bagga; Amy E. Pasquinelli

Palabras clave: Green Fluorescent Protein; miRNA Target; miRNA Gene; miRNA Precursor; Specific miRNAs.

Pp. 1-20

Dormancy and the Cell Cycle

Michael A. Campbell

The localization of the Kip-related proteins to meristematic regions suggests that these proteins might be associated with dormancy regulation. Additionally, the localization of chromatin remodeling to specific genes and loci may reveal the important regulation mechanisms for the dormant state. Developmental mutants that fail to develop bud scales and shift meristem programs toward an over-wintering bud would be informative in identifying. A substantial body of work has been accomplished in identifying genes and proteins associated with cell division and the cell cycle in plants. However, most of the recent research has focused on the annual A. thaliana as a model. In order to progress rapidly in the area of plant meristem dormancy, a model system has to be adopted. Many perennial species are slow growing, genetically complex, and have little background genetic research that can be used to support dormancy studies. Recent advances into the genetic structure of poplar and recent interest in some perennial relatives of A. thaliana may create opportunities for models systems.

Palabras clave: Cell Cycle; Plant Biol; Signal Transduction Mechanism; Dormant State; Leafy Spurge.

Pp. 21-33

The OPT Family Functions in Long-Distance Peptide and Metal Transport in Plants

Mark Lubkowitz

Palabras clave: Brassica Juncea; Schizosaccharomyces Pombe; Peptide Transport; Yellow Stripe; Oligopeptide Transporter.

Pp. 35-55

Phospholipid-Derived Signaling in Plant Response to Temperature and Water Stresses

Xuemin Wang

Palabras clave: Stomatal Closure; Cold Acclimation; Phosphatidic Acid; Freezing Tolerance; Stomatal Movement.

Pp. 57-66

Anionic Nutrient Transport in Plants: The Molecular Basis of the Sulfate Transporter Gene Family

Hideki Takahashi; Naoko Yoshimoto; Kazuki Saito

Palabras clave: Cystic Fibrosis Transmembrane Regulator; Sulfate Transporter; Sulfate Uptake; Sulfur Deficiency; Sulfur Limitation.

Pp. 67-80

Purification of Protein Complexes by Immunoaffinity Chromatography: Application to Transcription Machinery

Nancy E. Thompson; Debra Bridges Jensen; Jennifer A. Lamberski; Richard R. Burgess

Palabras clave: Propylene Glycol; Sigma Factor; Immunoaffinity Chromatography; Immunoaffinity Purification; Sigma Subunit.

Pp. 81-100

Iron Transport and Metabolism in Plants

Loubna Kerkeb; Erin L. Connolly

Palabras clave: Iron Deficiency; Iron Uptake; Iron Transport; Iron Limitation; Ferric Reductase.

Pp. 119-140

Salt Stress Signaling and Mechanisms of Plant Salt Tolerance

Viswanathan Chinnusamy; Jianhua Zhu; Jian-Kang Zhu

Palabras clave: Transgenic Plant; Salt Stress; Osmotic Stress; Salt Tolerance; Plant Cell Environ.

Pp. 141-177