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Nature Biotechnology
Resumen/Descripción – provisto por la editorial en inglés
Nature Biotechnology is a monthly journal covering the science and business of biotechnology. It publishes new concepts in technology/methodology of relevance to the biological, biomedical, agricultural and environmental sciences as well as covers the commercial, political, ethical, legal, and societal aspects of this research. The first function is fulfilled by the peer-reviewed research section, the second by the expository efforts in the front of the journal. We provide researchers with news about business; we provide the business community with news about research developments.Palabras clave – provistas por la editorial
No disponibles.
Disponibilidad
| Institución detectada | Período | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No detectada | desde jul. 2012 / hasta dic. 2023 | Nature.com |
Información
Tipo de recurso:
revistas
ISSN impreso
1087-0156
ISSN electrónico
1546-1696
Editor responsable
Springer Nature
País de edición
Reino Unido
Fecha de publicación
1996-
Cobertura temática
Tabla de contenidos
Modeling intercellular communication in tissues using spatial graphs of cells
David S. Fischer
; Anna C. Schaar; Fabian J. Theis
<jats:title>Abstract</jats:title><jats:p>Models of intercellular communication in tissues are based on molecular profiles of dissociated cells, are limited to receptor–ligand signaling and ignore spatial proximity in situ. We present node-centric expression modeling, a method based on graph neural networks that estimates the effects of niche composition on gene expression in an unbiased manner from spatial molecular profiling data. We recover signatures of molecular processes known to underlie cell communication.</jats:p>
Palabras clave: Cell Biology; Developmental Biology; Embryology; Anatomy.
Pp. No disponible
Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution
Qing Dai; Li-Sheng Zhang; Hui-Lung Sun; Kinga Pajdzik; Lei Yang; Chang Ye; Cheng-Wei Ju; Shun Liu; Yuru Wang; Zhong Zheng; Linda Zhang; Bryan T. Harada; Xiaoyang Dou; Iryna Irkliyenko; Xinran Feng; Wen Zhang; Tao Pan; Chuan He
<jats:title>Abstract</jats:title><jats:p>Functional characterization of pseudouridine (Ψ) in mammalian mRNA has been hampered by the lack of a quantitative method that maps Ψ in the whole transcriptome. We report bisulfite-induced deletion sequencing (BID-seq), which uses a bisulfite-mediated reaction to convert pseudouridine stoichiometrically into deletion upon reverse transcription without cytosine deamination. BID-seq enables detection of abundant Ψ sites with stoichiometry information in several human cell lines and 12 different mouse tissues using 10–20 ng input RNA. We uncover consensus sequences for Ψ in mammalian mRNA and assign different ‘writer’ proteins to individual Ψ deposition. Our results reveal a transcript stabilization role of Ψ sites installed by TRUB1 in human cancer cells. We also detect the presence of Ψ within stop codons of mammalian mRNA and confirm the role of Ψ in promoting stop codon readthrough in vivo. BID-seq will enable future investigations of the roles of Ψ in diverse biological processes.</jats:p>
Palabras clave: Cell Biology; Developmental Biology; Embryology; Anatomy.
Pp. No disponible
Absolute quantification of single-base m6A methylation in the mammalian transcriptome using GLORI
Cong Liu; Hanxiao Sun; Yunpeng Yi; Weiguo Shen; Kai Li; Ye Xiao; Fei Li; Yuchen Li; Yongkang Hou; Bo Lu; Wenqing Liu; Haowei Meng; Jinying Peng; Chengqi Yi; Jing Wang
Palabras clave: Cell Biology; Developmental Biology; Embryology; Anatomy.
Pp. No disponible
Targeting MYC with modular synthetic transcriptional repressors derived from bHLH DNA-binding domains
Thomas E. Speltz; Zeyu Qiao; Colin S. Swenson; Xianghang Shangguan; John S. Coukos; Christopher W. Lee; Deborah M. Thomas; Jesse Santana; Sean W. Fanning; Geoffrey L. Greene; Raymond E. Moellering
Palabras clave: Cell Biology; Developmental Biology; Embryology; Anatomy.
Pp. No disponible
Programmable eukaryotic protein synthesis with RNA sensors by harnessing ADAR
Kaiyi Jiang; Jeremy Koob; Xi Dawn Chen; Rohan N. Krajeski; Yifan Zhang; Verena Volf; Wenyuan Zhou; Samantha R. Sgrizzi; Lukas Villiger; Jonathan S. Gootenberg; Fei Chen; Omar O. Abudayyeh
Palabras clave: Cell Biology; Developmental Biology; Embryology; Anatomy.
Pp. No disponible
A split, conditionally active mimetic of IL-2 reduces the toxicity of systemic cytokine therapy
Alfredo Quijano-Rubio; Aladdin M. Bhuiyan; Huilin Yang; Isabel Leung; Elisa Bello; Lestat R. Ali; Kevin Zhangxu; Jilliane Perkins; Jung-Ho Chun; Wentao Wang; Marc J. Lajoie; Rashmi Ravichandran; Yun-Huai Kuo; Stephanie K. Dougan; Stanley R. Riddell; Jamie B. Spangler; Michael Dougan; Daniel-Adriano Silva; David Baker
<jats:title>Abstract</jats:title><jats:p>The therapeutic potential of recombinant cytokines has been limited by the severe side effects of systemic administration. We describe a strategy to reduce the dose-limiting toxicities of monomeric cytokines by designing two components that require colocalization for activity and that can be independently targeted to restrict activity to cells expressing two surface markers. We demonstrate the approach with a previously designed mimetic of cytokines interleukin-2 and interleukin-15—Neoleukin-2/15 (Neo-2/15)—both for <jats:italic>trans</jats:italic>-activating immune cells surrounding targeted tumor cells and for <jats:italic>cis</jats:italic>-activating directly targeted immune cells. In <jats:italic>trans</jats:italic>-activation mode, tumor antigen targeting of the two components enhanced antitumor activity and attenuated toxicity compared with systemic treatment in syngeneic mouse melanoma models. In <jats:italic>cis</jats:italic>-activation mode, immune cell targeting of the two components selectively expanded CD8<jats:sup>+</jats:sup> T cells in a syngeneic mouse melanoma model and promoted chimeric antigen receptor T cell activation in a lymphoma xenograft model, enhancing antitumor efficacy in both cases.</jats:p>
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Multifactorial profiling of epigenetic landscapes at single-cell resolution using MulTI-Tag
Michael P. Meers; Geneva Llagas; Derek H. Janssens; Christine A. Codomo; Steven Henikoff
<jats:title>Abstract</jats:title><jats:p>Chromatin profiling at locus resolution uncovers gene regulatory features that define cell types and developmental trajectories, but it remains challenging to map and compare different chromatin-associated proteins in the same sample. Here we describe Multiple Target Identification by Tagmentation (MulTI-Tag), an antibody barcoding approach for profiling multiple chromatin features simultaneously in single cells. We optimized MulTI-Tag to retain high sensitivity and specificity, and we demonstrate detection of up to three histone modifications in the same cell: H3K27me3, H3K4me1/2 and H3K36me3. We apply MulTI-Tag to resolve distinct cell types and developmental trajectories; to distinguish unique, coordinated patterns of active and repressive element regulatory usage associated with differentiation outcomes; and to uncover associations between histone marks. Multifactorial epigenetic profiling holds promise for comprehensively characterizing cell-specific gene regulatory landscapes in development and disease.</jats:p>
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
The Coronavirus Standards Working Group’s roadmap for improved population testing
Tim Mercer; Neil Almond; Michael A. Crone; Patrick S. G. Chain; Alina Deshpande; Deepa Eveleigh; Paul Freemont; Sebastien Fuchs; Russell Garlick; Jim Huggett; Martin Kammel; Po-E Li; Mojca Milavec; Elizabeth M. Marlowe; Denise M. O’Sullivan; Mark Page; Gary A. Pestano; Sara Suliman; Birgitte Simen; John J. Sninsky; Lynne Sopchak; Cristina M. Tato; Peter M. Vallone; Jo Vandesompele; Thomas J. White; Heinz Zeichhardt; Marc Salit
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Matthew T. N. Yarnall; Eleonora I. Ioannidi; Cian Schmitt-Ulms; Rohan N. Krajeski; Justin Lim; Lukas Villiger; Wenyuan Zhou; Kaiyi Jiang; Sofya K. Garushyants; Nathaniel Roberts; Liyang Zhang; Christopher A. Vakulskas; John A. Walker; Anastasia P. Kadina; Adrianna E. Zepeda; Kevin Holden; Hong Ma; Jun Xie; Guangping Gao; Lander Foquet; Greg Bial; Sara K. Donnelly; Yoshinari Miyata; Daniel R. Radiloff; Jordana M. Henderson; Andrew Ujita; Omar O. Abudayyeh; Jonathan S. Gootenberg
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Author Correction: m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome
Lulu Hu; Shun Liu; Yong Peng; Ruiqi Ge; Rui Su; Chamara Senevirathne; Bryan T. Harada; Qing Dai; Jiangbo Wei; Lisheng Zhang; Ziyang Hao; Liangzhi Luo; Huanyu Wang; Yuru Wang; Minkui Luo; Mengjie Chen; Jianjun Chen; Chuan He
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible