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Nature Biotechnology
Resumen/Descripción – provisto por la editorial en inglés
Nature Biotechnology is a monthly journal covering the science and business of biotechnology. It publishes new concepts in technology/methodology of relevance to the biological, biomedical, agricultural and environmental sciences as well as covers the commercial, political, ethical, legal, and societal aspects of this research. The first function is fulfilled by the peer-reviewed research section, the second by the expository efforts in the front of the journal. We provide researchers with news about business; we provide the business community with news about research developments.Palabras clave – provistas por la editorial
No disponibles.
Disponibilidad
| Institución detectada | Período | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No detectada | desde jul. 2012 / hasta dic. 2023 | Nature.com |
Información
Tipo de recurso:
revistas
ISSN impreso
1087-0156
ISSN electrónico
1546-1696
Editor responsable
Springer Nature
País de edición
Reino Unido
Fecha de publicación
1996-
Cobertura temática
Tabla de contenidos
High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs
Tony P. Huang; Zachary J. Heins
; Shannon M. Miller; Brandon G. Wong; Pallavi A. Balivada; Tina Wang; Ahmad S. Khalil; David R. Liu
<jats:title>Abstract</jats:title><jats:p>Despite the availability of Cas9 variants with varied protospacer-adjacent motif (PAM) compatibilities, some genomic loci—especially those with pyrimidine-rich PAM sequences—remain inaccessible by high-activity Cas9 proteins. Moreover, broadening PAM sequence compatibility through engineering can increase off-target activity. With directed evolution, we generated four Cas9 variants that together enable targeting of most pyrimidine-rich PAM sequences in the human genome. Using phage-assisted noncontinuous evolution and eVOLVER-supported phage-assisted continuous evolution, we evolved Nme2Cas9, a compact Cas9 variant, into variants that recognize single-nucleotide pyrimidine-PAM sequences. We developed a general selection strategy that requires functional editing with fully specified target protospacers and PAMs. We applied this selection to evolve high-activity variants eNme2-T.1, eNme2-T.2, eNme2-C and eNme2-C.NR. Variants eNme2-T.1 and eNme2-T.2 offer access to N<jats:sub>4</jats:sub>TN PAM sequences with comparable editing efficiencies as existing variants, while eNme2-C and eNme2-C.NR offer less restrictive PAM requirements, comparable or higher activity in a variety of human cell types and lower off-target activity at N<jats:sub>4</jats:sub>CN PAM sequences.</jats:p>
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Multiplexed, single-molecule, epigenetic analysis of plasma-isolated nucleosomes for cancer diagnostics
Vadim Fedyuk; Nir Erez; Noa Furth; Olga Beresh; Ekaterina Andreishcheva; Abhijeet Shinde; Daniel Jones; Barak Bar Zakai; Yael Mavor; Tamar Peretz; Ayala Hubert; Jonathan E. Cohen; Azzam Salah; Mark Temper; Albert Grinshpun; Myriam Maoz; Aviad Zick; Guy Ron; Efrat Shema
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Multi-omic single-cell velocity models epigenome–transcriptome interactions and improves cell fate prediction
Chen Li; Maria C. Virgilio; Kathleen L. Collins; Joshua D. Welch
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
The role of transposon inverted repeats in balancing drought tolerance and yield-related traits in maize
Xiaopeng Sun; Yanli Xiang; Nannan Dou; Hui Zhang; Surui Pei; Arcadio Valdes Franco; Mitra Menon; Brandon Monier; Taylor Ferebee; Tao Liu; Sanyang Liu; Yuchi Gao; Jubin Wang; William Terzaghi; Jianbing Yan; Sarah Hearne; Lin Li; Feng Li; Mingqiu Dai
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Precision mitochondrial DNA editing with high-fidelity DddA-derived base editors
Seonghyun Lee; Hyunji Lee; Gayoung Baek; Jin-Soo Kim
<jats:title>Abstract</jats:title><jats:p>Bacterial toxin DddA-derived cytosine base editors (DdCBEs)—composed of split DddA<jats:sub>tox</jats:sub> (a cytosine deaminase specific to double-stranded DNA), custom-designed TALE (transcription activator-like effector) DNA-binding proteins, and a uracil glycosylase inhibitor—enable mitochondrial DNA (mtDNA) editing in human cells, which may pave the way for therapeutic correction of pathogenic mtDNA mutations in patients. The utility of DdCBEs has been limited by off-target activity, which is probably caused by spontaneous assembly of the split DddA<jats:sub>tox</jats:sub> deaminase enzyme, independent of DNA-binding interactions. We engineered high-fidelity DddA-derived cytosine base editors (HiFi-DdCBEs) with minimal off-target activity by substituting alanine for amino acid residues at the interface between the split DddA<jats:sub>tox</jats:sub> halves. The resulting domains cannot form a functional deaminase without binding of their linked TALE proteins at adjacent sites on DNA. Whole mitochondrial genome sequencing shows that, unlike conventional DdCBEs, which induce hundreds of unwanted off-target C-to-T conversions in human mtDNA, HiFi-DdCBEs are highly efficient and precise, avoiding collateral off-target mutations, and as such, they will probably be desirable for therapeutic applications.</jats:p>
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Publisher Correction: Single-sequence protein structure prediction using a language model and deep learning
Ratul Chowdhury; Nazim Bouatta; Surojit Biswas; Christina Floristean; Anant Kharkar; Koushik Roy; Charlotte Rochereau; Gustaf Ahdritz; Joanna Zhang; George M. Church; Peter K. Sorger; Mohammed AlQuraishi
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Mathematical modeling of epigenetic gene regulation during cell differentiation
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Author Correction: Massively parallel phenotyping of coding variants in cancer with Perturb-seq
Oana Ursu; James T. Neal; Emily Shea; Pratiksha I. Thakore; Livnat Jerby-Arnon; Lan Nguyen; Danielle Dionne; Celeste Diaz; Julia Bauman; Mariam Mounir Mosaad; Christian Fagre; April Lo; Maria McSharry; Andrew O. Giacomelli; Seav Huong Ly; Orit Rozenblatt-Rosen; William C. Hahn; Andrew J. Aguirre; Alice H. Berger; Aviv Regev; Jesse S. Boehm
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Long-term preservation of liver grafts brings ‘off-the-shelf’ organs closer
Paulo N. Martins; Davide Ghinolfi
Palabras clave: Biomedical Engineering; Molecular Medicine; Applied Microbiology and Biotechnology; Bioengineering; Biotechnology.
Pp. No disponible
Mapping mRNA modifications for functional studies
Joshua D. Jones; Daniel E. Eyler; Kristin S. Koutmou
Palabras clave: Cell Biology; Developmental Biology; Embryology; Anatomy.
Pp. No disponible