Catálogo de publicaciones - revistas

<jats:title>Abstract</jats:title><jats:p>Plant cell walls are dynamic structures that play crucial roles in growth, development, and stress responses. Despite our growing understanding of cell wall biology, the connections between cell wall integrity (CWI) and cell cycle progression in plants remain poorly understood. This review aims to explore the intricate relationship between CWI and cell cycle progression in plants, drawing insights from studies in yeast and mammals. We provide an overview of the plant cell cycle, highlight the role of endoreplication in cell wall composition, and discuss recent findings on the molecular mechanisms linking CWI perception to cell wall biosynthesis and gene expression regulation. Furthermore, we address future perspectives and unanswered questions in the field, such as the identification of specific CWI sensing mechanisms and the role of CWI maintenance in the growth-defense trade-off. Elucidating these connections could have significant implications for crop improvement and sustainable agriculture.</jats:p>

<jats:title>Abstract</jats:title><jats:p>Cyanobacteria are oxygen-evolving photosynthetic prokaryotes that affect the global carbon and nitrogen turnover. <jats:italic>Synechocystis</jats:italic> sp. PCC 6803 (<jats:italic>Synechocystis</jats:italic> 6803) is a model cyanobacterium that has been widely studied and can utilize and uptake various nitrogen sources and amino acids from the outer environment and media. l-arginine is a nitrogen-rich amino acid used as a nitrogen reservoir in <jats:italic>Synechocystis</jats:italic> 6803, and its biosynthesis is strictly regulated by feedback inhibition. Argininosuccinate synthetase (ArgG; EC 6.3.4.5) is the rate-limiting enzyme in arginine biosynthesis and catalyzes the condensation of citrulline and aspartate using ATP to produce argininosuccinate, which is converted to l-arginine and fumarate through argininosuccinate lyase (ArgH). We performed a biochemical analysis of <jats:italic>Synechocystis</jats:italic> 6803 ArgG (<jats:italic>Sy</jats:italic>ArgG) and obtained a <jats:italic>Synechocystis</jats:italic> 6803 mutant overexpressing <jats:italic>Sy</jats:italic>ArgG and ArgH of <jats:italic>Synechocystis</jats:italic> 6803 (<jats:italic>Sy</jats:italic>ArgH). The specific activity of <jats:italic>Sy</jats:italic>ArgG was lower than that of other arginine biosynthesis enzymes and <jats:italic>Sy</jats:italic>ArgG was inhibited by arginine, especially among amino acids and organic acids. Both arginine biosynthesis enzyme-overexpressing strains grew faster than the wild-type <jats:italic>Synechocystis</jats:italic> 6803. Based on previous reports and our results, we suggest that <jats:italic>Sy</jats:italic>ArgG is the rate-limiting enzyme in the arginine biosynthesis pathway in cyanobacteria and that arginine biosynthesis enzymes are similarly regulated by arginine in this cyanobacterium. Our results contribute to elucidating the regulation of arginine biosynthesis during nitrogen metabolism.</jats:p>

<jats:title>Abstract</jats:title><jats:p>Knowing how chromosome recombination works is essential for plant breeding. It enables the design of crosses between different varieties to combine desirable traits and create new ones. This is because the meiotic crossovers between homologous chromatids are not purely random, and various strategies have been developed to describe and predict such exchange events. Recent studies have used methylation data to predict chromosomal recombination in rice using machine learning models. This approach proved successful due to the presence of a positive correlation between the CHH context cytosine methylation and recombination rates in rice chromosomes. This paper assesses the question if methylation can be used to predict recombination in four plant species: Arabidopsis, maize, sorghum, and tomato. The results indicate a positive association between CHH context methylation and recombination rates in certain plant species, with varying degrees of strength in their relationships. The CG and CHG methylation contexts show negative correlation with recombination. Methylation data was key effectively in predicting recombination in sorghum and tomato, with a mean determination coefficient of 0.65 ± 0.11 and 0.76 ± 0.05, respectively. In addition, the mean correlation values between predicted and experimental recombination rates were 0.83 ± 0.06 for sorghum and 0.90 ± 0.05 for tomato, confirming the significance of methylomes in both monocotyledonous and dicotyledonous species. The predictions for Arabidopsis and maize were not as accurate, likely due to the comparatively weaker relationships between methylation contexts and recombination, in contrast to sorghum and tomato, where stronger associations were observed. To enhance the accuracy of predictions, further evaluations using data sets closely related to each other might prove beneficial. In general, this methylome-based method holds great potential as a reliable strategy for predicting recombination rates in various plant species, offering valuable insights to breeders in their quest to develop novel and improved varieties.</jats:p>

<jats:title>Abstract</jats:title><jats:p>Arbuscular mycorrhizal symbiosis (AM) is a beneficial trait originating with the first land plants, which has subsequently been lost by species scattered throughout the radiation of plant diversity to the present day, including the model <jats:italic>Arabidopsis thaliana</jats:italic>. To explore if elements of this apparently beneficial trait are still present and could be reactivated we generated <jats:italic>Arabidopsis</jats:italic> plants expressing a constitutively active form of <jats:italic>Interacting Protein of DMI3</jats:italic>, a key transcription factor that enables AM within the Common Symbiosis Pathway, which was lost from <jats:italic>Arabidopsis</jats:italic> along with the AM host trait. We characterize the transcriptomic effect of expressing <jats:italic>IPD3</jats:italic> in <jats:italic>Arabidopsis</jats:italic> with and without exposure to the AM fungus (AMF) <jats:italic>Rhizophagus irregularis</jats:italic>, and compare these results to the AM model <jats:italic>Lotus japonicus</jats:italic> and its <jats:italic>ipd3</jats:italic> knockout mutant <jats:italic>cyclops-4</jats:italic>. Despite its long history as a non-AM species, restoring <jats:italic>IPD3</jats:italic> in the form of its constitutively active DNA-binding domain to <jats:italic>Arabidopsis</jats:italic> altered expression of specific gene networks. Surprisingly, the effect of expressing <jats:italic>IPD3</jats:italic> in <jats:italic>Arabidopsis</jats:italic> and knocking it out in <jats:italic>Lotus</jats:italic> was strongest in plants not exposed to AMF, which is revealed to be due to changes in <jats:italic>IPD3</jats:italic> genotype causing a transcriptional state, which partially mimics AMF exposure in non-inoculated plants. Our results indicate that molecular connections to symbiosis machinery remain in place in this nonAM species, with implications for both basic science and the prospect of engineering this trait for agriculture.</jats:p>

<jats:title>Abstract</jats:title><jats:p>SQUAMOSA PROMOTER BINDING PROTEIN-LIKEs (<jats:italic>SPLs</jats:italic>) encode plant-specific transcription factors that regulate plant growth and development, stress response, and metabolite accumulation. However, there is limited information on <jats:italic>Scutellaria baicalensis SPLs</jats:italic>. In this study, 14 <jats:italic>SbSPLs</jats:italic> were identified and divided into 8 groups based on phylogenetic relationships. <jats:italic>SbSPLs</jats:italic> in the same group had similar structures. Abscisic acid-responsive (ABRE) and MYB binding site (MBS) cis-acting elements were found in the promoters of 8 and 6 <jats:italic>SbSPLs</jats:italic>. Segmental duplications and transposable duplications were the main causes of <jats:italic>SbSPL</jats:italic> expansion. Expression analysis based on transcriptional profiling showed that <jats:italic>SbSPL1</jats:italic>, <jats:italic>SbSPL10</jats:italic>, and <jats:italic>SbSPL13</jats:italic> were highly expressed in roots, stems, and flowers, respectively. Expression analysis based on quantitative real-time polymerase chain reaction (RT‒qPCR) showed that most <jats:italic>SbSPLs</jats:italic> responded to low temperature, drought, abscisic acid (ABA) and salicylic acid (SA), among which the expression levels of <jats:italic>SbSPL7/9/10/12</jats:italic> were significantly upregulated in response to abiotic stress. These results indicate that <jats:italic>SbSPLs</jats:italic> are involved in the growth, development and stress response of <jats:italic>S. baicalensis</jats:italic>. In addition, 8 Sba-miR156/157 s were identified, and <jats:italic>SbSPL1-5</jats:italic> was a potential target of Sba-miR156/157 s. The results of target gene prediction and coexpression analysis together indicated that <jats:italic>SbSPLs</jats:italic> may be involved in the regulation of L-phenylalanine (L-Phe), lignin and jasmonic acid (JA) biosynthesis. In summary, the identification and characterization of the <jats:italic>SbSPL</jats:italic> gene family lays the foundation for functional research and provides a reference for improved breeding of <jats:italic>S. baicalensis</jats:italic> stress resistance and quality traits.</jats:p>