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Genetic Engineering of Mesenchymal Stem Cells

Jan A. Nolta (eds.)

Resumen/Descripción – provisto por la editorial

No disponible.

Palabras clave – provistas por la editorial

Cell Biology; Biomedicine general; Hematology; Transplant Surgery; Animal Physiology

Disponibilidad
Institución detectada Año de publicación Navegá Descargá Solicitá
No detectada 2006 SpringerLink

Información

Tipo de recurso:

libros

ISBN impreso

978-1-4020-3935-5

ISBN electrónico

978-1-4020-3959-1

Editor responsable

Springer Nature

País de edición

Reino Unido

Fecha de publicación

Información sobre derechos de publicación

© Springer Science+Business Media B.V. 2006

Tabla de contenidos

Mesenchymal Stem Cell Engineering and Transplantation

F. Aerts; G. Wagemaker

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 1-44

Establishment and Transduction of Primary Human Stromal/Mesenchymal Stem Cell Monolayers

T. Meyerrose; I. Rosova; M. Dao; P. Herrbrich; G. Bauer; J. Nolta

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 45-58

Gene Expression Profiles of Mesenchymal Stem Cells

D. G. Phinney

Past studies profiling gene expression in MSCs have typically revealed a preponderance of expressed transcripts encoding structural proteins common in skeletal tissue and secreted factors that regulate hematopoiesis. Our SAGE analysis of the MSC transcriptome corroborated many of these previous findings, but also revealed that the cells express a plethora of transcripts encoding proteins involved in cell communication, motility, neural activity, angiogenesis and other biological processes that characterize marrow stroma. Therefore, the heterogeneity of the MSC transcriptome appears to reflect the nature and function of the different cell types that comprise this organ, the complexity of which is under appreciated. Our studies also indicate that the transcriptome of a clonal MSC population is characterized by a high percentage of expressed transcripts encoding proteins of an indeterminate nature. These findings reflect a basic lack of knowledge regarding the biology of MSCs, which attributes to the difficulty in ascribing a molecular phenotype to these stem cells. Comparative genomics studies indicate that transcripts uniquely expressed in MSCs include transcription factors and signalling molecules involved in limb bud morphogenesis, thereby providing clues to the regulatory mechanisms governing self-renewal and lineage commitment of MSCs. Deciphering these molecular pathways will further our understanding of the nature and biology of this unique stem cell population.

Pp. 59-80

In Vivo Homing and Regeneration of Freshly Isolated and Cultured Murine Mesenchymal Stem Cells

R. E. Ploemacher

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 81-92

Non-Human Primate Models of Mesenchymal Stem Cell Transplantation

S. M. Devine; R. Hoffman

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 93-110

Engineering of Human Adipose-Derived Mesenchymal Stem-Like Cells

J. K. Fraser; M. Zhu; B. Strem; M. H. Hedrick

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 111-125

Uncommitted Progenitors in Cultures of Bone Marrow-Derived Mesenchymal Stem Cells

J. J. Minguell; A. Erices; W. D. Sierralta

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 127-133

Bone Marrow Mesenchymal Stem Cell Transplantation for Children with Severe Osteogenesis Imperfecta

E. M. Horwitz; P. L. Gordon

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 135-150

Clinical Trials of Human Mesenchymal Stem Cells to Support Hematopoietic Stem Cell Transplantation

O. N. Koç

In summary, adipose contains a population of cells that has extensive self-renewal capacity and the ability to differentiate along multiple lineages. The cells possessing this activity can be obtained in large numbers at high frequency from a tissue source that can be extracted in large quantities with minimal morbidity. These cells can be infected by adenoviral, oncoretroviral, and lentiviral vectors with moderate to high efficiency. Thus, adipose tissue appears to represent a potential clinically useful source of cells for cellular therapy and gene transfer applications.

Pp. 151-162