Catálogo de publicaciones - revistas

<jats:title>Abstract</jats:title><jats:sec> <jats:title>Main conclusion</jats:title> <jats:p>Genetic variation in seed protein composition, seed protein gene expression and predictions of seed protein physiochemical properties were documented in <jats:italic>C. sativa</jats:italic> and other <jats:italic>Camelina</jats:italic> species.</jats:p> </jats:sec><jats:sec> <jats:title>Abstract</jats:title> <jats:p>Seed protein diversity was examined in six <jats:italic>Camelina</jats:italic> species (<jats:italic>C. hispida, C. laxa</jats:italic>, <jats:italic>C. microcarpa</jats:italic>, <jats:italic>C. neglecta</jats:italic>, <jats:italic>C. rumelica</jats:italic> and <jats:italic>C. sativa</jats:italic>). Differences were observed in seed protein electrophoretic profiles, total seed protein content and amino acid composition between the species. Genes encoding major seed proteins (cruciferins, napins, oleosins and vicilins) were catalogued for <jats:italic>C. sativa</jats:italic> and RNA-Seq analysis established the expression patterns of these and other genes in developing seed from anthesis through to maturation. Examination of 187 <jats:italic>C. sativa</jats:italic> accessions revealed limited variation in seed protein electrophoretic profiles, though sufficient to group the majority into classes based on high MW protein profiles corresponding to the cruciferin region. <jats:italic>C. sativa</jats:italic> possessed four distinct types of cruciferins, named CsCRA, CsCRB, CsCRC and CsCRD, which corresponded to orthologues in <jats:italic>Arabidopsis thaliana</jats:italic> with members of each type encoded by homeologous genes on the three <jats:italic>C. sativa</jats:italic> sub-genomes. Total protein content and amino acid composition varied only slightly; however, RNA-Seq analysis revealed that <jats:italic>CsCRA</jats:italic> and <jats:italic>CsCRB</jats:italic> genes contributed &gt; 95% of the cruciferin transcripts in most lines, whereas <jats:italic>CsCRC</jats:italic> genes were the most highly expressed cruciferin genes in others, including the type cultivar DH55. This was confirmed by proteomics analyses. Cruciferin is the most abundant seed protein and contributes the most to functionality. Modelling of the <jats:italic>C. sativa</jats:italic> cruciferins indicated that each type possesses different physiochemical attributes that were predicted to impart unique functional properties. As such, opportunities exist to create <jats:italic>C. sativa</jats:italic> cultivars with seed protein profiles tailored to specific technical applications.</jats:p> </jats:sec>

<jats:title>Abstract</jats:title><jats:sec> <jats:title>Main conclusion</jats:title> <jats:p>This manuscript identifies cherry orthologues of genes implicated in the development of pericarpic fruit and pinpoints potential options and restrictions in the use of these targets for commercial exploitation of parthenocarpic cherry fruit.</jats:p> </jats:sec><jats:sec> <jats:title>Abstract</jats:title> <jats:p>Cherry fruit contain a large stone and seed, making processing of the fruit laborious and consumption by the consumer challenging, inconvenient to eat ‘on the move’ and potentially dangerous for children. Availability of fruit lacking the stone and seed would be potentially transformative for the cherry industry, since such fruit would be easier to process and would increase consumer demand because of the potential reduction in costs. This review will explore the background of seedless fruit, in the context of the ambition to produce the first seedless cherry, carry out an in-depth analysis of the current literature around parthenocarpy in fruit, and discuss the available technology and potential for producing seedless cherry fruit as an ‘ultimate snacking product’ for the twenty-first century.</jats:p> </jats:sec>

<jats:title>Abstract</jats:title><jats:sec> <jats:title>Main conclusions</jats:title> <jats:p><jats:italic>C. campestris</jats:italic> parasitisation increases internal host defences at the expense of environmentally directed ones in the host species <jats:italic>A. campestris</jats:italic>, thus limiting plant defence against progressive parasitisation.</jats:p> </jats:sec><jats:sec> <jats:title>Abstract</jats:title> <jats:p><jats:italic>Cuscuta campestris</jats:italic> Yunck is a holoparasitic species that parasitises wild species and crops. Among their hosts, <jats:italic>Artemisia campestris</jats:italic> subsp. <jats:italic>variabilis</jats:italic> (Ten.) Greuter is significantly affected in natural ecosystems. Limited information is available on the host recognition mechanism and there are no data on the interactions between these species and the effects on the primary and specialised metabolism in response to parasitisation. The research aims at evaluating the effect of host–parasite interactions, through a GC–MS untargeted metabolomic analysis, chlorophyll <jats:italic>a</jats:italic> fluorescence, ionomic and δ<jats:sup>13</jats:sup>C measurements<jats:italic>,</jats:italic> as well as volatile organic compound (VOC) fingerprint in <jats:italic>A. campestris</jats:italic> leaves collected in natural environment. <jats:italic>C. campestris</jats:italic> parasitisation altered plant water status, forcing stomatal opening, stimulating plant transpiration, and inducing physical damages to the host antenna complex, thus reducing the efficiency of its photosynthetic machinery. Untargeted-metabolomics analysis highlighted that the parasitisation significantly perturbed the amino acids and sugar metabolism, inducing an increase in the production of osmoprotectants, which generally accumulate in plants as a protective strategy against oxidative stress. Notably, VOCs analysis highlighted a reduction in sesquiterpenoids and an increase in monoterpenoids levels; involved in plant defence and host recognition, respectively. Moreover, <jats:italic>C. campestris</jats:italic> induced in the host a reduction in 3-hexenyl-acetate, a metabolite with known repellent activity against <jats:italic>Cuscuta</jats:italic> spp. We offer evidences that <jats:italic>C. campestris</jats:italic> parasitisation increases internal host defences via primary metabolites at the expense of more effective defensive compounds (secondary metabolites), thus limiting <jats:italic>A. campestris</jats:italic> defence against progressive parasitisation.</jats:p> </jats:sec>