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Péptidos bioactivos producidos por bacterias lácticas con actividad inhibitoria del desarrollo de microorganismos

Diana María Müller Georgina Guadalupe Tonarelli Miriam Cristina Strumia Ana María Strasser de Saad Carlos Antonio Zalazar Arturo Carlos Simonetta

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Resumen/Descripción – provisto por el repositorio digital
Lactobacillus plantarum LP31 strain, isolated from Argentinian dry-fermented sausages, produced an bactericidal substance active against some spoilage and foodborne pathogenic bacteria. It was preliminarily characterized as a bacteriocin and was sequencially purified by solid phase extraction, gel filtration chromatography and reverse phase. We have found that this bacteriocin is a peptide with approximately fourteen aminoacids and have molecular weight of 1558.85 Da. Plantaricin-149 is a bacteriocin produced by Lactobacillus plantarum NRIC. In this work, a synthetic peptide analog denoted as Pln149a and a hybrid peptide (HP) with the N-terminal amino acid sequence os Pediocin PA-1 was prepared by solid phase. The antagonistic activity against gram-positive and gram-negative bacteria was tested, and the secondary structure was studied by Circular Dichroism (CD) and Fluorescence Spectroscopy, under several conditions. Plna was inhibitory against Staphylococcus aureus and Listeria strains. The HP and its C-terminal part, was active only against Listeria strains, in accordance with the results found for Pln149a. Analysis of secondary structure, found that Pln149a acts via the carpet mechanism. According to the results obtained with the hybrid bacteriocin we conclude that the molecule contains two domains: a cationic, N-terminal β-sheet domain that mediates binding of the bacteriocin to the target cell surface and an amphipathic C-terminal α-helical domain that possibly may be involved in the penetration into the hydrophobic part of the target cell membrane.
Palabras clave – provistas por el repositorio digital

Bacteriocim; Circular dichroism; Fluorescence; Bacteriocina; Dicroismo cicular; Fluorescencia

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Institución detectada Año de publicación Navegá Descargá Solicitá
No requiere 2007 Biblioteca Virtual de la Universidad Nacional del Litoral (SNRD) acceso abierto

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Tipo de recurso:

tesis

Idiomas de la publicación

  • español castellano

País de edición

Argentina

Fecha de publicación