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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.

The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project” approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction.

*Cover basic concepts and techniques used in molecular biology research labs
*Student-tested labs proven successful in a real classroom laboratories
*Exercises simulate a cloning project that would be performed in a real research lab
*"Project" approach to experiments gives students an overview of the entire process
*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation.
Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid vector of large DNA segments, and the mating system of bacilli. Molecular cloning session covers complementation system and dominance analyses in Bacillus, genetic fusion of Escherichia coli lac genes to a Bacillus subtilis promoter, and DNA cloning of B. subtilis. It also describes the construction of trimethoprim resistant B. subtilis plasmid and expression of E. coli trp genes cloned in B. subtilis. Session III encompasses chapters that discuss protein secretion by bacilli; regulation of alpha-amylase production in B. subtilis; entomocidal toxin translation of B. thuringiensis; and expression of crystal protein, heterologous, and eukaryotic genes in bacilli. Session IV focuses on various aspects of DNA metabolism of bacilli, such as the interaction of bacterial chromosome with cell membrane; plasmid DNA in competent cells and protoplasts of B. subtilis; analysis of peptides synthesized by B. subtilis mutants; and DNA repair, uptake, restriction, modification, and recombination. The final session examines species-specific translation, control of gene expression and replications in plasmids, development of expression-vector in B. subtilis, and regulatory modifications of RNA polymerase. Each chapter is presented in an experimental manner, consisting of a summary of the study, materials and methods, results, as well as references.

Molecular Control of Proliferation and Differentiation documents the proceedings of the 35th symposium of The Society for Developmental Biology entitled "Molecular Control of Proliferation and Differentiation," held at Asilomar, Monterey Peninsula, California, June 8-11, 1976. The contributions made by researchers at the symposium are organized into seven parts. Part I presents a paper on cell communication in embryological development. Part II includes studies on growth factors, including fibroblast growth factor and epidermal growth factor. Part III examines the factors affecting nerve cell differentiation and function. It includes papers on nerve growth factor and peptides as central nervous system neurotransmitters. Part IV focuses on cell interactions in blood cell development. It includes studies on factors affecting the differentiation of blood cells and the proliferation of hemopoietic stem cells in vitro. Part V examines cell interactions in the immune system. Part VI deals with cell interactions in organogenesis. Part VII covers the factors effecting differentiation in lower eukaryotes.

Molecular Cytology presents an integrated version about the morphology and biochemistry of the cell. This two-volume book focuses on the dynamic aspects of cytology and on the nucleocytoplasmic interactions in unicellular organisms and eggs.
The first chapter covers the history of cell, cytology, and nucleic acids, as well as the uniformity and diversity in cell. The book then discusses various methods used in cell biology, including optical, cytochemical, biological, biochemical, and biophysical techniques. It also examines the activities of cytoplasm and nucleus during interphase. The final chapter describes various phases of the cell cycle, the structure of metaphase chromosomes, the molecular organization of the mitotic apparatus, and the cytokinesis, with emphasis on the main mitotic abnormalities.
With the aim of linking the morphology and biochemistry of the cell, this book is intended for advanced students, research workers, biochemists, and cytologists who wish to broaden their knowledge in cell.