Catálogo de publicaciones

Laboratory Methods in Vesicular and Vectorial Transport describes the procedures used to study the mechanisms of vesicular transport along the secretory and endocytic paths, including electron microscopy, autoradiography, and methods associated with cyto- and immunocytochemistry, genetics, and biochemistry. It investigates vectorial transport to the cisternal space of the rough endoplasmic reticulum (ER), as well as protein translocation across the ER, strategies for gaining access to the cytoplasm, cell-free analysis of vesicle fusion, the structure of glycoproteins, and the use of cell systems for analysis of vesicular traffic.
Organized into seven parts encompassing 20 chapters, this volume begins with an overview of protein topology in the ER and the use of cross-linking methods to probe the molecular environment of translocating polypeptide chains. It then discusses the reconstitution of secretory protein translocation from detergent-solubilized rough microsomes; the use of anti-idiotype antibodies to characterize protein-protein interactions; the use of perforated cells to elucidate intracellular membrane transport; delivery of macromolecules into cells expressing a viral membrane fusion protein; and digitonin permeabilization procedures for studying endosome acidification and function. The reader is also introduced to reconstitution of intracellular vesicle fusion in a cell-free system after receptor-mediated endocytosis; immunoisolation using magnetic solid supports; endosome and lysosome purification by free-flow electrophoresis; remodeling of glycoprotein oligosaccharides after endocytosis; and replica plating of animal cells.
This book will interest students, researchers, geneticists, biochemists, and cell biologists.

Laboratory Primate Handbook deals with the proper care and handling, treatment, and transportation of nonhuman primates to be used as research subjects in a laboratory environment. It considers the protection of human and animal health, identification, procurement, and husbandry, as well as compliance with federal regulations.
Organized into 10 chapters, this volume begins with an overview of living primates and their classification, followed by procurement and production for biomedical research purposes. It then discusses housing and care of primates; preventive medicine and public health programs aimed at minimizing the hazards that may arise from diseases transmissible between nonhuman primates and man; and restraint and special techniques, including anesthetics and pre-anesthetics. The book also covers nutrition for primates and prevention of nutritional diseases, as well as generalized infectious diseases; parasites that cause disease in primates; and collection of physiological data on primates. Finally, the book looks at federal regulations and policies governing the humane care and treatment of nonhuman primates used in research.
This book will benefit biomedical researchers involved in laboratory studies of nonhuman primates.

Laboratory Safety: Theory and Practice focuses on theoretical aspects of the hazards the students, technicians, and scientists encounter in the laboratory. It presents methods of risk assessment that can be applied to technologies as they are translated from the scientist’s mind to the laboratory bench. It is organized into three sections designated as General Laboratory Safety, Biological Laboratory Safety, and Medical and Psychological Factors.
The first section, encompassing three chapters, discusses hazards found in almost all laboratories; pertinent safety theories and practices; ubiquitous compounds that are either toxic or carcinogenic and guidelines for their use; and radiation hazards. Chapters 4 to 7 focus on the safety in the biological laboratory. Discussions on relatively complex group of viruses, approach to recombinant DNA research, and awareness on the possible hazards associated with the field are included in this book. Chapters 6 and 7 present design and function of biohazard laboratories and the hazards relating to laboratory animals. The final section discusses medical surveillance of persons at risk and the psychological factors involved in accident control. It presents a comprehensive list of chemical agents, their sources, subsequent physical effects, and the accepted mode of medical surveillance. Various genetic screening tests and their potential use for the evaluation of presumptive and actual mutagens are also covered.
This book is ideal for safety and design engineers, students, technicians, and scientists.

Lacquer Chemistry and Applications explores the topic of lacquer, the only natural product polymerized by an enzyme that has been used for a coating material in Asian countries for thousands of years.

Although the human-lacquer-culture, including cultivation of the lacquer tree, harvesting, and the use of lacquer sap, has a long history of more than thousand years, there is very little information available on the modern scientific methods to study lacquer chemistry.

This book, based on the results of the authors' 30 years of research on lacquer chemistry, offers lacquer researchers a unique reference on the science and applications of this extremely important material.

• Covers the chemistry and properties of lacquer, including synthesis of its various components
• Provides up-to-date analytical techniques for lacquer identification and characterization
• Discusses possible toxicity effects
• Outlines new modification techniques for developing higher performance material
• Presents the history of this versatile coating material that has evolved from its origins in Asian countries over thousands of years

This reference text provides students and practicing engineers with the theoretical knowledge and practical skills needed to identify, model, and solve structural analysis problems involving continuous fiber laminated composites. The principles are illustrated throughout with numerous examples and case studies, as well as example problems similar in nature to those found in strength of materials texts. A solutions manual is available. Extensive coverage of test methods and experimental techniques distinguished Staab from the many theory-led books on composites, making it ideal for practicing engineers and courses with a practical emphasis.

The second edition of Laminar Composites is ideal for engineers with a firm understanding of basic structural analysis discovering for the first time the intricacies of orthotropic material behavior and laminate analysis. The fundamental equations required to formulate and assess the behavior of laminated composites are presented in an easy to follow format.

Revised and updated throughout, the second edition also includes three new chapters; beams, plates, shells, each covering aspects such as bending, deformation and vibration accompanied by the relevant equations of equilibrium and motion.

• Tutorial style ideal for self-study or use on strength of materials courses (undergraduate and graduate – online solutions manual available)
• A foundational reference work for a class of composite materials of growing commercial importance
• Coverage of test methods and experimental techniques distinguished Staab from the many theory-led books on composites, making it ideal for practicing engineers and courses with a practical emphasis