Catálogo de publicaciones

Gene Manipulations in Fungi combines a review of classical fungal genetics, contemporary research, and responsible speculation about the future.
This book focuses on yeasts and molds; because yeast is the primary model system for eukaryotes and that there is an elegant research on molds. The applications of fungi, including their economic importance, are addressed. The book emphasizes the need for improved transformation systems, appropriate vectors, and broadly applicable selectable markers in this field of interest.
This book will help stimulate the development of innovative approaches in this subject matter.

Gene Probes for Bacteria focuses on the preparation and use of nucleic acid probes for identifying bacteria in clinical specimens and in other samples of practical or scientific interest.
Organized into 17 chapters, this book addresses which nucleic acid probes are available; how and when to utilize them; what to expect in terms of results obtained with their use; and how to prepare probes. Each chapter is composed of comprehensive manuscript covering different microorganisms. A few bacterial species are treated in more than one chapter to provide a multidimensional picture of important microbes, and to compile the knowledge gained from different laboratories using the same or different probes or methods.
This reference material will be of great use to professionals, technicians, and R&D directors in universities, federal and state-dependent service and research institutes, and private and industrial laboratories.

Gene Regulation documents the proceedings of the CETUS-UCLA Symposium "Gene Regulation," held in Keystone, Colorado in March/April 1982. The symposium related gene structure and regulatory sequences to overall genomic organization and genetic evolution. It was the first meeting to focus on regulation of eukaryotic gene expression since the maturation in recombinant DNA technology.
The book is organized into four parts. Part I presents studies on the structure of eukaryotic genes, including the organization and molecular basis for differential expression of the mouse λ light chain genes; globin gene transcription and RNA processing; and the cloning of the human chromosomal α1-antitrypsin gene and its structural comparison with the chicken gene coding for ovalbumin. Part II on chromatin structure includes papers on nuclease sensitivity of the ovalbumin gene and its flanking DNA sequences; and the relationship of chromatin structure to DNA sequence. Part III on gene expression includes papers on the role of poly(A) in eukaryotic mRNA metabolism and the in vitro transcription of Drosophila tRNA genes. Part IV on cellular biology includes studies such as the importance of calmodulin to the eukaryotic cells.

Gene therapy is emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability of DNA in the physiological milieu limits its therapeutic potential, hence a vector which can protect and efficiently transport DNA to the target cells must be developed. Nanotechnology-based non-viral vectors have been proposed as potential candidates. Various polymeric nanoparticles have been shown to be suitable, with high cellular uptake efficiencies and reduced cytotoxicity. These delivery vectors form condensed complexes with DNA which result in shielding against enzymatic degradation and enhanced cellular targeting. Advantages including easy manipulatibility, high stability, low cost and high payload, mean that nanoparticles from various polymers have been exploited. Gene therapy gives a systematic account of the many aspects of nanotechnology mediated gene therapy, from the preparation of nanoparticles to physicochemical characterization, and follows with applications in in vitro and in vivo models. This book emphasizes the various aspects of nanotechnology-based gene therapy, with initial chapters detailing the tools and techniques available for preparation and in vitro and in vivo characterization of nanoparticles. Later chapters provide exhaustive details on polymeric systems employed for gene therapy.

• Provides an overview of nanotechnology applications in gene therapy, from preparation of nanoparticles to in vitro and in vivo studies
• Details the tools and techniques available for preparation, characterization and in vitro and in vivo study of nanoparticles
• Details the limitations of nanoparticle-mediated gene therapy and proposes ways in which they may be overcome

Gene Therapy for Viral Infections provides a comprehensive review of the broader field of nucleic acid and its use in treating viral infections. The text bridges the gap between basic science and important clinical applications of the technology, providing a systematic, integrated review of the advances in nucleic acid-based antiviral drugs and the potential advantages of new technologies over current treatment options. 

Coverage begins with the fundamentals, exploring varying topics, including harnessing RNAi to silence viral gene expression, antiviral gene editing, viral gene therapy vectors, and non-viral vectors.

Subsequent sections include detailed coverage of the developing use of gene therapy for the treatment of specific infections, the principles of rational design of antivirals, and the hurdles that currently face the further advancement of gene therapy technology.

• Provides coverage of gene therapy for a variety of infections, including HBV, HCV, HIV, hemorrhagic fever viruses, and respiratory and other viral infections
• Bridges the gap between the basic science and the important medical applications of this technology
• Features a broad approach to the topic, including an essential overview and the applications of gene therapy, synthetic RNA, and other antiviral strategies that involve nucleic acid engineering
• Presents perspectives on the future use of nucleic acids as a novel class of antiviral drugs
• Arms the reader with the cutting-edge information needed to stay abreast of this developing field