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El objetivo general de la tesis es contribuir al conocimiento de la vegetación sammófila del centro-oeste de la Argentina mediante el análisis geosinfitosociológico (florístico y sinecológico) (paisaje vegetal) de la vegetación de los Médanos Grandes–San Juan, uno de los sistemas eólicos más importantes de Argentina. El análisis realizado permite definir en el sistema dos subambientes, el dominado por procesos eólicos, con megadunas, y el dominado por procesos fluvio-eólicos, sin megadunas. El sistema, en general estabilizado y fijo, tiene actividad sólo en las crestas con escasa cobertura vegetal. La vegetación juega un papel determinante en la dinámica de la arena y en el modelado de este sistema, estando estrechamente relacionada con la disponibilidad de agua que se ajusta a tres modelos: -el de escurrimiento superficial y subsuperficial desde la bajada pedemontana de la sierra de Pie de Palo al norte, -el de la freática relacionada con los ríos San Juan, al oeste, y Bermejo, al este, y – el del agua de lluvia que en las megadunas determina un bulbo húmedo con contenidos de 1,21-2,4 g de agua/100 g de arena, entre los 15-35 m de profundidad, aprovechada por las raíces de los arbustos. Cuatro comunidades vegetales dominan en el sistema:-el pastizal de Panicum urvilleanum en las crestas, el -matorral de Tricomaria usillo-Bulnesia retama en las laderas de las dunas e intermédanos a más de 690 m, el -matorral de Atriplex lampa en los sectores marginales con suelos salinos y el -bosque de Prosopis flexuosa en los intermédanos bajos. Sintaxonómicamente la vegetación pertenece a tres Clases, la Panico urvilleani-Sporoboletea rigentis Esk., 1992 en ambientes sammófilos, la Suaedetea divaricatae Alonso et Conticello ex Martinez Carretero, 2001 en ambientes halófilos y la Larreetea divaricato-cuneifoliae Roig, 89 en la estepa arbustiva del Monte. La relación entre las comunidades vegetales y las asociaciones geomorfológicas permite establecer dos paisajes: el Paisaje I o de Sistema eólico que incluye el 55 % de la superficie y el Paisaje II o de Sistema fluvio-eólico. Palabras claves: eólico, megadunas, comunidades vegetales, dinamismo, bioclima, paisaje vegetal.

El reloj circadiano controla los ritmos comportamentales, fisiológicos y metabólicos en todos los organismos en los que se lo ha estudiado. Este oscilador, cuya periodicidad es cercana a las 24 hs, se mantiene sincronizado gracias a diversas claves ambientales. En Drosophila, como en otros organismos, se han identificado algunos de los componentes responsables de generar y mantener las oscilaciones moleculares. Asimismo se han caracterizado circuitos neuronales en los que componentes del reloj fluctúan con un período cercano a 24 hs. A lo largo de esta tesis se estudió el rol de la vía de bone morphogenetic protein (BMP) en Drosophila en el circuito neuronal que controla el comportamiento rítmico. Esta vía de señalización, además de estar involucrada en la organogénesis y el crecimiento, funciona como una señal sináptica retrógrada involucrada en la sinaptogénesis, la morfología sináptica y el control de la homeostasis en Drosophila y en otros organismos. La alteración de esta vía en el circuito PDF en estadios adultos conlleva un alargamiento del período endógeno de la actividad locomotora, que correlaciona con el ritmo de acumulación y localización subcelular de period, un componente central del reloj molecular. Los resultados obtenidos a lo largo de este trabajo permiten proponer que la vía de BMP es capaz de modular la trascripción de genes centrales del reloj molecular y que schnurri podría constituir un regulador negativo determinante del nivel de expresión de los genes reloj en Drosophila.

Laboratory Animal Anaesthesia, Fourth Edition provides a basic guide to anaesthesia for a very diverse audience needing content, with straight-forward, structured style of writing. Updated with effects of anaesthetics in different laboratory species, including sources of dose rates will be incorporated into tabular material. New information on pain assessment and pain management will be covered, and an increased emphasis on rats and mice for anaesthesia and perioperative care. With newly revised, full color illustrations to facilitate best learning, Laboratory animal Anesthesia, Fourth Edition provides procedures, key points and invaluable advice from a well-known and respected veterinary anesthetist and scientist with over 30 years of experience in the field.

• Written by a veterinary anesthetist and scientist with over 30 years' experience in the field, and who is actively engaged in research in this area
• Focuses on procedures involving rats and mice used in research
• Provides those with limited experience of anesthesia with the information they need to carry our procedures effectively, safely, and humanely, as well as those with more experience to continue a career with laboratory animal model research
• Includes rapid, easily accessed information using tabulated summaries

Laboratory Animal Anesthesia looks at recent significant developments in anesthetic practices in laboratory experiments involving animals. It also provides information about basic standards for proper use of anesthesia. In addition, it examines the equipment and different anesthetic agents that are used in performing an experiment on animals. The book also discusses the profound effects of anesthesia on the physiological aspect of the animals’ body systems, such as hypothermia and respiratory depression. The book addresses the proper management and care that should be provided for the animals that undergo anesthesia. Furthermore, it covers different anesthetic procedures that should be used on various kinds of small animals intended for laboratory experiments.
The main goal of this book is to provide information about the different anesthetic agents used in experiments, and the proper standards to follow when using anesthetics on lab animals.

• New edition provides new information on anesthesia and analgesia, and has an extensively revised and updated bibliography
• Provides a balanced consideration of the needs of scientific research and the welfare of laboratory animals
• Written by a veterinary anesthetist and scientist with over 30 years' experience in the field, and who is actively engaged in research in this area
• Provides rapid, easily accessed information using tabulated summaries
• Provides those with limited experience of anesthesia with the information they need to carry our procedures effectively, safely, and humanely
• Provides sufficient depth for the more experienced anesthetist moving to this field

Laboratory Animal Medicine, Third Edition, is a fully revised publication from the American College of Laboratory Medicine’s acclaimed blue book series. It presents an up-to-date volume that offers the most thorough coverage of the biology, health, and care of laboratory animals.

The book is organized by species, with new inclusions of chinchillas, birds, and program and employee management, and is written and edited by known experts in the fields.

Users will find gold-standard guidance on the study of laboratory animal science, as well as valuable information that applies across all of the biological and biomedical sciences that work with animals.

• Organized by species for in-depth understanding of biology, health, and best care of animals
• Features the inclusion of chinchillas, quail, and zebra finches as animal models
• Offers guidance on program and employee management
• Covers regulations, policies, and laws for laboratory animal management worldwide

Laboratory Animal Welfare provides a comprehensive, up-to-date look into the new science of animal welfare within laboratory research. Animals specifically considered include rodents, cats and dogs, nonhuman primates, agricultural animals, avian animals and aquatic animals.

The book examines the impact of experiment design and environment on animal welfare, as well as emergency situations and euthanasia practices. Readers will benefit from a review of regulations and policy guidelines concerning lab animal use, as well as information on assessing animal welfare. With discussions of the history and ethics of animals in research, and a debate on contemporary and international issues, this book is a go-to resource for laboratory animal welfare.

Laboratory Animals: Regulations and Recommendations for Global Collaborative Research is the only publication to offer a compilation of standards across the world in the care, welfare and use of animals in research. Timely in the new legislation in numerous regions of the world, this book provides the information in easily accessible, readable language.

For professionals across laboratory animal science and biomedical research, Laboratory Animals: Regulations and Recommendations for Global Collaborative Research provides a broad picture of the regulations required in other areas of the world and is essential to appropriately manage animal care and use programs.

• Offers a worldwide view and global compilation of regulations, guidelines and recommendations for laboratory animal research
• Saves valuable time researching different regional legislation and regulations
• Provides insight into factors that play roles in the regulatory framework for countries and geographic regions
• Written in "layman’s" terms to easily understand legislation and regulations

Laboratory Methods in Vesicular and Vectorial Transport describes the procedures used to study the mechanisms of vesicular transport along the secretory and endocytic paths, including electron microscopy, autoradiography, and methods associated with cyto- and immunocytochemistry, genetics, and biochemistry. It investigates vectorial transport to the cisternal space of the rough endoplasmic reticulum (ER), as well as protein translocation across the ER, strategies for gaining access to the cytoplasm, cell-free analysis of vesicle fusion, the structure of glycoproteins, and the use of cell systems for analysis of vesicular traffic.
Organized into seven parts encompassing 20 chapters, this volume begins with an overview of protein topology in the ER and the use of cross-linking methods to probe the molecular environment of translocating polypeptide chains. It then discusses the reconstitution of secretory protein translocation from detergent-solubilized rough microsomes; the use of anti-idiotype antibodies to characterize protein-protein interactions; the use of perforated cells to elucidate intracellular membrane transport; delivery of macromolecules into cells expressing a viral membrane fusion protein; and digitonin permeabilization procedures for studying endosome acidification and function. The reader is also introduced to reconstitution of intracellular vesicle fusion in a cell-free system after receptor-mediated endocytosis; immunoisolation using magnetic solid supports; endosome and lysosome purification by free-flow electrophoresis; remodeling of glycoprotein oligosaccharides after endocytosis; and replica plating of animal cells.
This book will interest students, researchers, geneticists, biochemists, and cell biologists.

Laboratory Primate Handbook deals with the proper care and handling, treatment, and transportation of nonhuman primates to be used as research subjects in a laboratory environment. It considers the protection of human and animal health, identification, procurement, and husbandry, as well as compliance with federal regulations.
Organized into 10 chapters, this volume begins with an overview of living primates and their classification, followed by procurement and production for biomedical research purposes. It then discusses housing and care of primates; preventive medicine and public health programs aimed at minimizing the hazards that may arise from diseases transmissible between nonhuman primates and man; and restraint and special techniques, including anesthetics and pre-anesthetics. The book also covers nutrition for primates and prevention of nutritional diseases, as well as generalized infectious diseases; parasites that cause disease in primates; and collection of physiological data on primates. Finally, the book looks at federal regulations and policies governing the humane care and treatment of nonhuman primates used in research.
This book will benefit biomedical researchers involved in laboratory studies of nonhuman primates.