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Cepas autóctonas de Lactobacillus aisladas de neonatos santafesinos para el desarrollo de alimentos probióticos: Aspectos tecnológicos. Herramientas moleculares para su detección en alimentos

Verónica Gregoret Binetti Ana Marta Susana Carrasco María Cristina Lurá Sergio Enrique Pasteris Vinderola Gabriel

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Resumen/Descripción – provisto por el repositorio digital
Specific strains should only be regarded as probiotics if they fulfill certain criteria: safety, technological and functional. The aim of this work was to study, from a comprehensive point of view (in vitro and in vivo tests) three Lactobacillus strains (L. paracasei JP1, L. rhamnosus 64 and L. gasseri 37) isolated from feces of local new-borns, determining some parameters of technological, biological and functional relevance. All strains were able to adequately growth in different economic culture media (cheese whey, buttermilk and milk), that were also suitable as cryoprotectants. As selective media, LP-MRS was more effective than B-MRS for the enumeration of all strains. The strains were resistant to different technological (refrigerated storage in acidified milk, frozen storage, salt tolerance) and biological (simulated gastrointestinal digestion, bile exposure) challenges. L. rhamnosus 64 and L. gasseri 37 were sensible to chloramphenicol, erythromycin, streptomycin, tetracycline and vancomycin and they increased the phagocytic activity of peritoneal macrophage and induced the proliferation of IgA producing cells in small intestine when administered to mice. Even when clinical trails are still needed, both strains fulfilled the main criteria proposed by FAO/WHO to considerer them as potential probiotics for the formulation of new foods for the local market.
Palabras clave – provistas por el repositorio digital

probiotics; Lactobacillus; technological characterization; functional characterization; probioticos; caracterización tecnológica; caracterización funcional

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No requiere 2012 Biblioteca Virtual de la Universidad Nacional del Litoral (SNRD) acceso abierto

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tesis

Idiomas de la publicación

  • español castellano

País de edición

Argentina

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