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Small-scale Freshwater Toxicity Investigations: Toxicity Test Methods
Christian Blaise ; Jean-François Férard (eds.)
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Institución detectada | Año de publicación | Navegá | Descargá | Solicitá |
---|---|---|---|---|
No detectada | 2005 | SpringerLink |
Información
Tipo de recurso:
libros
ISBN impreso
978-1-4020-3119-9
ISBN electrónico
978-1-4020-3120-5
Editor responsable
Springer Nature
País de edición
Reino Unido
Fecha de publicación
2005
Información sobre derechos de publicación
© Springer 2005
Cobertura temática
Tabla de contenidos
Overview of Contemporary Toxicity Testing
Christian Blaise; Jean-François Férard
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 1. - Introduction | Pp. 1-68
Microtox® Acute Toxicity Test
B. Thomas Johnson
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 69-105
Solid-Phase Test for Sediment Toxicity Using the Luminescent Bacterium,
Ken Doe; Paula Jackman; Rick Scroggins; Don McLeay; Gary Wohlgeschaffen
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 107-136
Algal Microplate Toxicity Test
Christian Blaise; Paule Vasseur
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 137-179
Algal Toxicity Test
Jane P. Staveley; Jerry C. Smrchek
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 181-202
Microalgal Toxicity Tests Using Flow Cytometry
Jennifer Stauber; Natasha Franklin; Merrin Adams
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 203-241
Algal Microplate Toxicity Test Suitable for Heavy Metals
Hans G. Peterson; Niels Nyholm; Norma Ruecker
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 243-270
Growth Inhibition Test
Mary Moody; Jennifer Miller
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 271-298
Spirotox Test — Acute Toxicity Test
Grzegorz Nałęcz-jawecki
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 299-322
Rotifer Ingestion Test for Rapid Assessment of Toxicity
Terry W. Snell
Aquatic ecosystems produce about 50% of the global biomass and play an important role in atmospheric carbon dioxide cycling. Since the primary producers are confined to the euphotic zone for energetic reasons, they are simultaneously exposed to short wavelength radiation. Solar UV affects growth, reproduction, photosynthetic production and many other physiological processes. Cyanobacteria are important ubiquitous prokaryotes which populate terrestrial and aquatic habitats. They account for up to 40 % of the marine biomass production and are important components of wet land ecosystems such as rice paddy fields. These organisms are also highly impaired by solar UV, but they and other motile microorganisms have developed mitigating strategies to protect themselves from this stress. One protection strategy is based on vertical migrations within the water column or a microbial mats. However, both motility and orientation are impaired by UV radiation. Another means of protection is achieved by the production of screening pigments including mycosporine-like amino acids (MAA) or scytonemins. MAAs are also produced by phytoplankton and macroalgae. In several organisms action spectra were measured which indicate that MAA synthesis is induced by UV in most cases. These sunscreen pigments prevent short wavelength radiation from reaching the UV sensitive DNA where it induces thymine dimers. Remaining dimers are removed by photorepair which involves the enzyme photolyase. The photosynthetic apparatus is another main target in primary aquatic biomass producers. Inhibition of the photosynthetic electron transport chain can be determined by oxygen measurements or by pulse amplitude modulated (PAM) fluorescence. Plants reduce the potentially deleterious effects of solar UV by decreasing the photosynthetic electron transport in photosystem II, a process called photoinhibition. Despite the dramatic effects of even ambient solar UV on individual species and physiological responses, the effect of ozone depletion on whole ecosystems is surprisingly low and close to the noise level induced by all other environmental factors such as mixing layer depth, cloud cover and temperature.
Part 2. - Toxicity test methods | Pp. 323-335