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Título de Acceso Abierto
Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice
Fatma Sarsu Abdelbagi M.A. Ghanim Priyanka Das Rajeev N. Bahuguna Paul Mbogo Kusolwa Muhammed Ashraf Sneh L. Singla-Pareek Ashwani Pareek Brian P. Forster Ivan Ingelbrecht
Resumen/Descripción – provisto por la editorial
No disponible.
Palabras clave – provistas por la editorial
Plant Breeding/Biotechnology; Agriculture
Disponibilidad
| Institución detectada | Año de publicación | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No requiere | 2018 | SpringerLink |
|
Información
Tipo de recurso:
libros
ISBN impreso
978-3-319-77337-7
ISBN electrónico
978-3-319-77338-4
Editor responsable
Springer Nature
País de edición
Reino Unido
Fecha de publicación
2018
Información sobre derechos de publicación
© International Atomic Energy Agency 2018
Cobertura temática
Tabla de contenidos
Correction to: Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice
Fatma Sarsu
The book was inadvertently published with several mistakes, which have now been corrected.
Pp. E1-E2
General Introduction
Fatma Sarsu
Breeding heat tolerant rice is one of the strategies to develop crop adaptation to the effect of climate change, particularly in major rice growing regions that are vulnerable to increased temperature. Developing high temperature tolerant rice varieties is already an important breeding target for several national/international breeding programs; however, changing weather patterns have increased the urgency to develop heat stress tolerant rice varieties, particularly varieties that are already well adapted to local environments. Mutation breeding is an effective approach to develop heat stress tolerance in crops, including rice. Therefore, rice mutation breeding for adaptation to high temperature can augment current technology to maintain crop yields.
Pp. 1-7
Screening Protocols for Heat Tolerance in Rice at the Seedling and Reproductive Stages
Fatma Sarsu
The methods include a protocol for screening heat tolerance of rice at the seedling stage; young seedlings were exposed to heat stress and the test takes 4–5 weeks and involves the visual scoring of symptoms which allows hundreds of seedlings to be evaluated in a short time. The visual screening method was extensively validated through laboratory, glasshouse, and field-based experiments. We also adapted a protocol for screening heat tolerant mutant lines at the flowering (reproductive) stage that has been specifically adjusted for a mutation breeding program. Here, plants were treated from the first day of anthesis at different temperatures and spikelet fertility at maturity was determined as a parameter to assess the heat tolerance of the selected genotypes.
Pp. 9-24
Validation of Screening Protocols for Heat Tolerance in Rice
Fatma Sarsu
Selected heat tolerant mutant rice genotypes were tested for physiological and biochemical indicators associated with the pre-field screen protocols. These tests included measuring physiological and biochemical indicators associated with plant stress responses, such as electrolyte leakage, malondialdehyde level, total protein content, antioxidant enzyme activity at seedling, vegetative stages and flowering stages to understand the mechanism of the heat tolerance characteristics/traits of the selected germplasm and explore the potential of pyramiding different mutations for durable heat tolerance. The candidate heat tolerant mutant lines were also tested in hot spot areas in the different countries.
Pp. 25-32
Conclusion
Fatma Sarsu
In conclusion, the comparison of candidate mutant rice lines with controls (standards) using various physiological and biochemical indicators showed that the selected heat tolerant mutant lines exhibited less electrolyte leakage and reduced levels of MDA, both indicative of improved plant performance under stress.
Pp. 33-34