Catálogo de publicaciones - revistas
Applied Spectroscopy
Resumen/Descripción – provisto por la editorial en inglés
Applied Spectroscopy is one of the world's leading spectroscopy journals, publishing high-quality articles, both fundamental and applied, covering all aspects of spectroscopy. Established in 1951, the journal is owned by the Society for Applied Spectroscopy and is published monthly. The journal is dedicated to fulfilling the mission of the Society to “…advance and disseminate knowledge and information concerning the art and science of spectroscopy and other allied sciences.” All manuscripts are rigorously peer-reviewed.Palabras clave – provistas por la editorial
No disponibles.
Disponibilidad
| Institución detectada | Período | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No detectada | desde ene. 1999 / hasta dic. 2023 | SAGE Journals |
Información
Tipo de recurso:
revistas
ISSN impreso
0003-7028
ISSN electrónico
1943-3530
Editor responsable
SAGE Publishing (SAGE)
País de edición
Estados Unidos
Fecha de publicación
1946-
Cobertura temática
Tabla de contenidos
Fatty Acid Determination in Human Milk Using Attenuated Total Reflection Infrared Spectroscopy and Solvent-Free Lipid Separation
Christopher K. Akhgar
; Vanessa Nürnberger; Marlene Nadvornik; Victoria Ramos-Garcia; Isabel Ten-Doménech; Julia Kuligowski; Andreas Schwaighofer; Erwin Rosenberg; Bernhard Lendl
<jats:p> This study introduces the first mid-infrared (IR)–based method for determining the fatty acid composition of human milk. A representative milk lipid fraction was obtained by applying a rapid and solvent-free two-step centrifugation method. Attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy was applied to record absorbance spectra of pure milk fat. The obtained spectra were compared to whole human milk transmission spectra, revealing the significantly higher degree of fatty acid–related spectral features in ATR FT-IR spectra. Partial least squares (PLS)–based multivariate regression equations were established by relating ATR FT-IR spectra to fatty acid reference concentrations, obtained with gas chromatography–mass spectrometry (GC-MS). Good predictions were achieved for the most important fatty acid sum parameters: saturated fatty acids (SAT, R<jats:sup>2</jats:sup><jats:sub>CV</jats:sub> = 0.94), monounsaturated fatty acids (MONO, R<jats:sup>2</jats:sup><jats:sub>CV</jats:sub> = 0.85), polyunsaturated fatty acids (PUFA, R<jats:sup>2</jats:sup><jats:sub>CV</jats:sub> = 0.87), unsaturated fatty acids (UNSAT, R<jats:sup>2</jats:sup><jats:sub>CV</jats:sub> = 0.91), short-chain fatty acids (SCFA, R<jats:sup>2</jats:sup><jats:sub>CV</jats:sub> = 0.79), medium-chain fatty acids (MCFA, R<jats:sup>2</jats:sup><jats:sub>CV</jats:sub> = 0.97), and long-chain fatty acids (LCFA, R<jats:sup>2</jats:sup><jats:sub>CV</jats:sub> = 0.88). The PLS selectivity ratio (SR) was calculated in order to optimize and verify each individual calibration model. All mid-IR regions with high SR could be assigned to absorbances from fatty acids, indicating high validity of the obtained models. </jats:p>
Pp. 730-736