Catálogo de publicaciones - tesis
Título de Acceso Abierto
Modelado de la electroforesis capilar por zona: Aplicaciones bioquímicas y biotecnológicas
María Virginia Piaggio Julio Alcides Deiber Nora Vizioli María Fernanda Silva Carlos Bregni Carlos Dante Brondino
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Resumen/Descripción – provisto por el repositorio digital
This Thesis presents a theoretical-computational model of capillary zone electrophoresis (CZE) involving small charged molecules, which is validated with experimental data. This model (non-ideal and linear) provides quantitative predictions of practical interest for electropherogram interpretations involving an analyte or a mixture of analytes. It is possible to extend this basic model (yielding the so-called extended models: LLCEM and PLLCEM) to more complex analytes like peptides and globular proteins, by including the charge regulation phenomenon, which leads one to relevant conclusions on the calculations of net charge, hydrodynamic radius, shape, hydration, pH near molecule and the estimation of pK variation of ionizing groups. Special emphasis is placed on the fact that these parameters are physically coupled. Also, the extended models may be useful to study the denaturizing process and conformational stability of globular proteins under the effect of different denaturant agents, from the measurement of simple electrophoretic mobilities as obtained from CZE. This Thesis shows that CZE, apart from being an analytical tool widely used, has a high power for the estimation of physicochemical properties like, for instance, hydrodynamic radius, diffusion coefficient, effective electrical charge, zeta potential and shape and hydration of analytes, when the electropherogram is modeled through the balance equations of transport phenomena coupled to electrokinetic effects.Palabras clave – provistas por el repositorio digital
Capillary electrophoresis; Computational modeling; Charge regulation phenomenon; Proteins; Peptides; Amino acids; Electroforesis capilar; Modelo computacional; Fenómeno de regulación de cargas; Proteínas; Péptidos; Aminoácidos
Disponibilidad
| Institución detectada | Año de publicación | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No requiere | 2007 | Biblioteca Virtual de la Universidad Nacional del Litoral (SNRD) |
|
Información
Tipo de recurso:
tesis
Idiomas de la publicación
- español castellano
País de edición
Argentina
Fecha de publicación
2007-11-28
