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<jats:sec><jats:title>Abstract</jats:title><jats:p>High concentrations of carnauba waxes (CRWs) that can compromise organoleptic properties are required to create self‐sustained and functional oleogels. The weak physical properties and stability of 4% w/w CRW–rice bran oil (RBO) oleogel were addressed by substituting CRW with beeswax (BW) in different weight ratios. The texture profile analyzer revealed that substituting only 10% (weight ratio) of CRW with BW improved the hardness compared to the mono‐CRW oleogel. The hardness of binary oleogels increased gradually as the proportion of BW increased. At a BW ratio of 70% or more, the hardness was three times higher than that of mono‐BW oleogel. Rheology analysis showed the same trend as the large deformation test; however, the hardest binary oleogels had lower critical strain and yield point compared to the mono‐wax oleogels, implying that they are more prone to lose their structure upon applied stress. Nevertheless, nearly all binary mixtures (except for 10%BW90%CRW) showed oil‐binding capacities above 99%, suggesting improved nucleation and crystallization process. Polarized light microscopy showed the coexistence of BW and CRW crystals and changes in the size and arrangement of wax crystals upon proportional changes of the two waxes. X‐ray diffraction confirmed no differences in the peaks’ location, and all oleogels had β′ polymorphism. Differential scanning calorimetry showed eutectic melting behavior in some binary blends. Oxidation stability in the binary wax oleogels improved as compared to the mono‐wax oleogel and bulk RBO. BW and CRW mixtures have promising oil‐structuring abilities and have various properties at different ratios that have the potential to be used as solid fat substitutes.</jats:p></jats:sec><jats:sec><jats:title>Practical Application</jats:title><jats:p>As a trending green oil‐structuring technology, oleogelation has shown great potential to reduce saturated fats in food systems. The current research provides valuable fundamental information on the strong synergistic interactions between beeswax and carnauba wax that have the potential to be used as solid fat substitutes created with a much lower total concentration of the required wax. This will help create wax oleogels with better organoleptic properties and less negative waxy mouthfeel. Such knowledge could prove beneficial for the development of healthy products that have potential applications in meat, bakery, dairy, pharmaceutical, as well as cosmetic industries.</jats:p></jats:sec>

<jats:sec><jats:title>Abstract</jats:title><jats:p>This study aimed to evaluate the anti‐cervical cancer activity of chondroitin sulfate–functionalized selenium nanoparticles (SeCS) and to elucidate their action mechanism. Cytotoxic effect of SeCS on HeLa cells was assessed by MTT assay. Further molecular mechanism of SeCS was analyzed by flow cytometric assay and western blotting. The results showed that treatment with SeCS resulted in a dose‐ and time‐dependent inhibition in the proliferation of HeLa cells. The data obtained from flow cytometry demonstrated that SeCS inhibited HeLa cell growth via the induction of S‐phase arrest and cell apoptosis. Further mechanism analysis found that SeCS down‐regulated expression levels of cyclin A and CDK2 and up‐regulated p21 expression, which contributed to S arrest. Moreover, SeCS increased the level of Bax and decreased the expression of Bcl‐2, resulting in the release of cytochrome C from mitochondria and activating caspase‐3/8/9 for caspase‐dependent apoptosis. Meanwhile, intracellular reactive oxygen species (ROS) levels were elevated after SeCS treatment, suggesting that ROS might be upstream of SeCS‐induced S‐phase arrest and cell apoptosis. These data show that SeCS has anti‐tumor effects and possesses the potential to become a new therapeutic agent or adjuvant therapy for cancer patients.</jats:p></jats:sec><jats:sec><jats:title>Practical Application</jats:title><jats:p>In our previous study, we used chondroitin sulfate to stabilize nano‐selenium to obtain SeCS to improve the bioactivity and stability of nano‐selenium. We found that it possessed an inhibitory effect on HeLa cells. However, the molecular mechanism remains unclear. This study elucidated the mechanism of SeCS damage to HeLa cells. SeCS has the potential to become a new therapeutic agent or adjuvant therapy for cancer patients.</jats:p></jats:sec>