Catálogo de publicaciones - revistas
Mycoses
Resumen/Descripción – provisto por la editorial en inglés
The journal Mycoses provides an international forum for original papers in English on the pathogenesis, diagnosis, therapy, prophylaxis, and epidemiology of fungal infectious diseases in humans and animals as well as on the biology of pathogenic fungi. Prominent mycoses of the skin have always been the domain of dermatology. Otorhinolaryngologists, gastroenterologists, and gynecologists are confronted with mycoses of the mucous membranes.Medical mycology as part of medical microbiology is advancing rapidly. Effective therapeutic strategies are already available in chemotherapy and are being further developed. Their application requires reliable laboratory diagnostic techniques, which, in turn, result from mycological basic research. Opportunistic mycoses vary greatly in their clinical and pathological symptoms, because the underlying disease of a patient at risk decisively determines their symptomatology and progress. Therefore, case histories are highly informative. Short communications on mycological topics complete the original articles. The journal mycoses is, therefore, of interest to scientists in fundamental mycological research, mycological laboratory diagnosticians, clinicians in all fields, and veterinarians.
Palabras clave – provistas por la editorial
pathogenesis; diagnosis; therapy; prophylaxis; epidemiology; fungal; infectious; disease; human; ani
Disponibilidad
| Institución detectada | Período | Navegá | Descargá | Solicitá |
|---|---|---|---|---|
| No detectada | desde ene. 1957 / hasta dic. 2023 | Wiley Online Library |
Información
Tipo de recurso:
revistas
ISSN impreso
0933-7407
ISSN electrónico
1439-0507
Editor responsable
John Wiley & Sons, Inc. (WILEY)
País de edición
Reino Unido
Fecha de publicación
1988-
Cobertura temática
Tabla de contenidos
doi: 10.1111/myc.13618
Development of single‐tube real‐time PCR assay for the rapid detection of Aspergillus and Fusarium—The two most common causative agents in fungal keratitis
Yamini Tawde; Sourav Das; Amit Gupta; Savitri Sharma
; Soham Basak; Twishi Shrimali; Shreya Singh; Shivaprakash M. Rudramurthy; Harsimran Kaur; Anup Ghosh
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>To compare the performance of conventional, semi‐nested and real‐time panfungal ITS PCRs for diagnosing fungal keratitis (FK) and develop genus‐specific real‐time PCR for the most common aetiology of FK.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>This multicentric study includes 232 corneal samples from suspected FK patients from four centres across India between November 2019 through August 2021. A total of 87 corneal buttons were included for the comparison of conventional, semi‐nested and real‐time ITS PCRs, of which 68 were from confirmed FK patients. Of these 87 samples, 44 (microscopy and culture positive for <jats:italic>Aspergillus</jats:italic> sp. and/or <jats:italic>Fusarium</jats:italic> sp.) were used for the standardisation of genus‐specific real‐time primers/probes. Subsequently, the best method showing highest sensitivity and specificity was validated in 188 samples.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>On Bayesian comparison, conventional ITS2 PCR showed best performance (sensitivity and specificity of 55.88% and 100%, respectively). Since, real‐time ITS2 PCR was also considerably efficient (sensitivity and specificity of 51.47% and 84.21%, respectively) in comparison with the conventional PCR but faster, cost‐effective, and less labor‐intensive, ITS‐2 real‐time PCR is a suitable method that can be applied along with culture and microscopy. During validation, real‐time PCR with genus‐specific primers showed 61.76% and 91.18% sensitivity with specificity of 98.05% and 79.22%, respectively, for <jats:italic>Aspergillus</jats:italic> sp. and <jats:italic>Fusarium</jats:italic> sp. <jats:italic>Aspergillus</jats:italic> probe, <jats:italic>Fusarium</jats:italic> probe and duplex PCR showed sensitivity of 52.94%, 50% and 54.41% with specificity of 92.86%, 82.47% and 75%, respectively. No cross‐reactivity of genus‐specific PCRs was observed during standardisation.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>ITS‐2 real‐time PCR can be applied as an adjunct with conventional methods for the diagnosis of FK. The genus‐specific duplex real‐time PCRs are rapid which reduces the turnaround time (TAT) avoiding the need for sequencing.</jats:p></jats:sec>
Palabras clave: Infectious Diseases; Dermatology; General Medicine.
Pp. 801-809