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<jats:p>The aim of this study was to identify the chemical composition of the Piper arboreumAubl. essential oil (EO), and to evaluate its inhibitory activity in vitroagainst the enzymes butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE). The EO was obtained by steam distillation of the leaves, which were collected in Pindal canton of the Loja province in southern Ecuador. The chemical composition was analyzed using the gas chromatography technique coupled to a mass spectrometry detector (GC-MS) and gas chromatography coupled to a flame ionization detector (GC-FID). A total of 41 compounds were identified, the major components found in the oil were limonene (31,46%), β-selinene (12,01%), (E)-caryophyllene (7,53%), bicyclogermacrene (6,72%), germacrene D (3,83%) and β-elemene (3,63%). In in vitro analyzes, the EO showed high selective inhibition for BuChE with an IC50inhibition value of 29,3±3,3 μg/mL. By contrast, the EO was not active against the AChE enzyme (IC50was 100,1±15,2 μg/mL).</jats:p>

<jats:p>The present review was aimed at documenting medicinal uses of species of the Euphorbiaceae family in Zimbabwe. Literature was collected from online databases such as BioMed Central, Web of Science, Springerlink, Google Scholar, Scielo, PubMed, Science Direct, ACS Publications, Scopus and JSTOR. This study showed that 29 species are used to manage human and animal diseases in Zimbabwe. These species are used against 49 medical conditions, mainly as ethnoveterinary medicine (21 use reports), and traditional medicine against respiratory (23 use reports), gastro-intestinal (14 use reports), male reproductive (11 use reports), antenatal and postpartum (10 use reports each) and sexually transmitted infections (9 use reports). Acalypha brachiata, Bridelia cathartica, B. mollis, Crotonmegalobotrys, Euphorbia ingens, E. matabelensis, Flueggea virosa, Monadenium lugardiae, Pseudolachnostylis maprouneifolia, Ricinus communisand Spirostachys africanahave the highest number of medicinal uses. There is need to unravel the therapeutic potential of the family through further ethnopharmacological research.</jats:p>

<jats:p>India's commercial advancement and development depend heavily on agriculture. A common fruit grown in tropical settings is citrus. A professional judgment is required while analyzing an illness because different diseases have slight variations in their symptoms. In order to recognize and classify diseases in citrus fruits and leaves, a customized CNN-based approach that links CNN with LSTM was developed in this research. By using a CNN-based method, it is possible to automatically differentiate from healthier fruits and leaves and those that have diseases such fruit blight, fruit greening, fruit scab, and melanoses. In terms of performance, the proposed approach achieves 96% accuracy, 98% sensitivity, 96% Recall, and an F1-score of 92% for citrus fruit and leave identification and classification and the proposed method was compared with KNN, SVM, and CNN and concluded that the proposed CNN-based model is more accurate and effective at identifying illnesses in citrus fruits and leaves</jats:p>

<jats:p>To study the effect of 50% ethanol extract of Bougainvillea xbuttiana on the enzymatic activity, cell viability and cytokine production provoked by the venom of Bothrops jararacain macro-phages. Three assays were used to study the effects of B. xbuttiana extract on the damage produced by B. jararaca: Enzymatic activity was detected by measuring the proteolytic and phos-pholipase A2; macrophages cytotoxicity was determined by the MTT method; levels of cytokine were evaluated using ELISA and a biological assay. After treatment with 300 μg/mL B. xbuttiana extract for 30 min, the proteolytic and phospholipase A2 activities of the venom were reduced to 95 and 61%, respectively. In macrophages cultures treated with B. xbuttiana extract combined with venom, the production of TNF-α, IL-6 and IFN-γ was reduced, whereas IL-10 was potentiated. Our results support the potential effect of the B. xbuttiana extract as a complementary therapy against the toxicity caused by the venom of B. jararaca snakes.</jats:p>

<jats:p>Cancer cells modify lipid metabolism to proliferate, Passiflora edulis (P. edulis) fruit juice (ZuFru) has antitumor activity, but whether a mechanism is through modulation of cell lipids is unknown. To establish if ZuFru modifies cholesterol and triglycerides in SW480 and SW620. ZuFru composition was studied by phytochemical march; antiproliferative activity by sulforhodamine B, cholesterol, and triglycerides by Folch method. Zufru contains anthocyanins, flavonoids, alkaloids, and tannins. Cell lines showed differences in their growth rate (p=0.049). At 39.6 μg/mL of ZuFru, cell viability was decreased: SW480 (45.6%) and SW620 (45.1%). In SW480, cholesterol (44.6%) and triglycerides (46.5%) decreased; In SW620, cholesterol decreased 14.8% and triglycerides increased 7%,with significant differences for both lines. Antiproliferative activity of ZuFru could be associated with the inhibition of intracellular biosynthesis of cholesterol and triglycerides in SW480. Action mechanisms need to be further investigated</jats:p>

<jats:p>The present study evaluated the efficacy of the mineralizing action of Casearia sylvestris ethanolic extract on bovine dentin blocks in its pure form and in dental paste, through scanning electron microscopy. The dentin blocks were immersed in artificial saliva and incubated at 37°C for 7 days. Subsequently, six groups were treated with different test substances and analysed qualitatively and quantitatively at 30 and 60 days. The tests used were Kruskal-Wallis and Dunn's. Shapiro-Wilk and ANOVA. The qualitative analysis at 30 days showed a difference between the groups treated with ethanolic extract and toothpaste. Quantitatively, at 30 days, treatment with ethanolic extract of Casearia showed a greater number of open dentinal tubules. At 60 days, the difference persisted only for the blocks treated with toothpaste. The results obtained indicated that there is a positive relationship between the use of Casearia sylvestris and obliteration of dentinal tubules.</jats:p>

<jats:p>Eclipta prostrata Linn. is an annual herbaceous plant used in traditional medicine, commonly known as “Trailing Eclipta” or False Daisy in English, ‘Keekirindiya’ in Sinhala and ‘Kayyantakara’ in Tamil. The aim of this study was to conduct a detailed pharmacognostical evaluation of E. prostrata found in Sri Lanka. Anatomical, physicochemical, phytochemical studies, and quantification of phyto-constituents were performed as per WHO guidelines. Whole plant was sequentially extracted into solvents with different polarities. Phytochemical screening and Thin Layer Chromatography (TLC) fingerprinting were carried out. Anatomical study and powder microscopy revealed useful diagnostic features. Physicochemical parameters such as moisture content, ash values (total, acid insoluble, water soluble) and extractable matter in water and ethanol were evaluated. Phytochemical screening and TLC fingerprinting revealed the presence of different types of phyto-constituents. Alkaloid, tannin, saponin, total flavonoid and total polyphenol contents were quantified. In conclusion, pharmacognostical study aids in establishing the standardization parameters of E.prostrata found in Sri Lanka.</jats:p>

<jats:p>The use and knowledge of native and naturalized medicinal plants were evaluated in four communities of Villa Corzo, Chiapas, Mexico. The information was obtained through semi-structured surveys conducted with 118 people of different age and gender categories. To determine the most important plant species, indexes of value and frequency of use were generated. Seventy-nine species of 49 families were recorded, as well as their use in the treatment of various diseases according to local knowledge. The species with the highest value of use were Aloe vera (L.) Burm. f., Verbena officinalis L. (verbena), Ocimum basilicum L. (basil) and Psidium guajava L. (guava). (guava). The most important families by number of species used were, in order of importance: Asteraceae and Rutaceae with five species Lamiaceae, Fabaceae and Rosaceae with four species each.</jats:p>

<jats:p>The aim of the present study was to assess the impacts of roasting and the type of extraction solvent (ethanol or water) on the hypolipidemic activity of xoconostle fruit peel extracts in a tyloxapol-induced model of hyperlipidemia. Water and ethanol extracts from raw and roasted Opuntia joconostle peels were obtained to quantify the phytochemicals contained within and assess their hypolipidemic activity in rats (n=5) against tyloxapol-induced dyslipidemia (400 mg/kg). The raw ethanol and water extracts, as well as the roasted water extract (200 mg/kg), showed hypolipidemic activity in the tyloxapol-treated group (p&lt;0.05). In contrast, the roasted sample extracted with ethanol did not show this effect. The concentrations of phenolic compounds (39.80 mg GAE/g) and flavonoids (16.42 ±0.14 mg QE/g) were higher in the ethanolic extracts than in the aqueous extracts. Conversely, the concentration of betalains (115.51 ±1.66 mg/100 g) was higher in the water extracts than in the ethanol extracts. It was concluded that the roasting process modified the concentration of some phytochemicals and their antioxidant capacity in vitro, producing a hypolipidemic effect in tyloxapol-induced hyperlipidemic rats.</jats:p>

<jats:p>We evaluated the effect of the total macerate (TM) and seed oil (SO) of mature Carica candamarcensisfruits, on the release of Matrix metalloproteinase 9 (MMP9) and the phosphorylation of MAPK in neutrophils. The antioxidant capacity of these extracts was evaluated by ABTS assay. Neutrophils stimulated with different dilutions of TM or SO were analyzed for cytotoxicity, MMP9 release, and MAPK phosphorylation, using trypan blue exclusion assays, zymography, and immunoblotting, respectively. Both extracts show antioxidant activity, being higher in TM; none presented cytotoxic effect. The 5% and 2.5% dilutions of TM significantly reduced MMP9 release, and all decreased MAPK phosphorylation. SO significantly increased the release of MMP9 and MAPK phosphorylation, the effect being greater when they were prestimulated with lipopolysaccharide.TM may have anti-inflammatory potential, while SO could have a priming effect that needs to be confirmed.</jats:p>