Catálogo de publicaciones - revistas

<jats:p>Much progress has been made recently in single-cell chromosome conformation capture technologies. However, a method that allows simultaneous profiling of chromatin architecture and gene expression has not been reported. Here, we developed an assay named “Hi-C and RNA-seq employed simultaneously” (HiRES) and performed it on thousands of single cells from developing mouse embryos. Single-cell three-dimensional genome structures, despite being heavily determined by the cell cycle and developmental stages, gradually diverged in a cell type–specific manner as development progressed. By comparing the pseudotemporal dynamics of chromatin interactions with gene expression, we found a widespread chromatin rewiring that occurred before transcription activation. Our results demonstrate that the establishment of specific chromatin interactions is tightly related to transcriptional control and cell functions during lineage specification.</jats:p>

<jats:p> Aging is characterized by a decline in tissue function, but the underlying changes at cellular resolution across the organism remain unclear. Here, we present the Aging Fly Cell Atlas, a single-nucleus transcriptomic map of the whole aging <jats:italic>Drosophila</jats:italic> . We characterized 163 distinct cell types and performed an in-depth analysis of changes in tissue cell composition, gene expression, and cell identities. We further developed aging clock models to predict fly age and show that ribosomal gene expression is a conserved predictive factor for age. Combining all aging features, we find distinctive cell type–specific aging patterns. This atlas provides a valuable resource for studying fundamental principles of aging in complex organisms. </jats:p>

<jats:p> Neuronal activity drives alterations in gene expression within neurons, yet how it directs transcriptional and epigenomic changes in neighboring astrocytes in functioning circuits is unknown. We found that neuronal activity induces widespread transcriptional up-regulation and down-regulation in astrocytes, highlighted by the identification of <jats:italic>Slc22a3</jats:italic> as an activity-inducible astrocyte gene that encodes neuromodulator transporter Slc22a3 and regulates sensory processing in the mouse olfactory bulb. Loss of astrocytic Slc22a3 reduced serotonin levels in astrocytes, leading to alterations in histone serotonylation. Inhibition of histone serotonylation in astrocytes reduced the expression of γ-aminobutyric acid (GABA) biosynthetic genes and GABA release, culminating in olfactory deficits. Our study reveals that neuronal activity orchestrates transcriptional and epigenomic responses in astrocytes while illustrating new mechanisms for how astrocytes process neuromodulatory input to gate neurotransmitter release for sensory processing. </jats:p>

<jats:p>Vulnerabilities of the world’s least developed countries to health, economic, and environmental crises were recently in the spotlight at a United Nations (UN) conference in Qatar. In March, the Doha Programme of Action was announced as a call for the developed world to renew commitments to support low- and middle-income countries in overcoming major challenges. UN Secretary General António Guterres aptly stated there are “no more excuses.” Such a commitment requires Global North-South and South-South partnerships that harness science and technology and that also empower the South to sustain progress. As a scientist from the Global South who can attest to science’s transformative role in this region of the world, individuals and organizations across sectors and society can play a strong role in supporting such a commitment.</jats:p>

<jats:p>New national lab would go to a historically Black institution</jats:p>

<jats:p>Some scientists worry reporting requirement is burdensome—and even insulting</jats:p>

<jats:p>Data on shark populations in coral reefs raise concern and hope for recovery</jats:p>

<jats:p>A stable organometallic compound with a Be-Be bond has been synthesized</jats:p>

<jats:p>Careful bias management and data fidelity are key</jats:p>

<jats:p>A biologist explores the cat’s origins, evolution, and complicated history with humans</jats:p>