Catálogo de publicaciones - revistas

<jats:p>Protein domains of low sequence complexity do not fold into stable, three-dimensional structures. Nevertheless, proteins with these sequences assist in many aspects of cell organization, including assembly of nuclear and cytoplasmic structures not surrounded by membranes. The dynamic nature of these cellular assemblies is caused by the ability of low-complexity domains (LCDs) to transiently self-associate through labile, cross-β structures. Mechanistic studies useful for the study of LCD self-association have evolved over the past decade in the form of simple assays of phase separation. Here, we have used such assays to demonstrate that the interactions responsible for LCD self-association can be dictated by labile protein structures poised close to equilibrium between the folded and unfolded states. Furthermore, missense mutations causing Charcot-Marie-Tooth disease, frontotemporal dementia, and Alzheimer’s disease manifest their pathophysiology in vitro and in cultured cell systems by enhancing the stability of otherwise labile molecular structures formed upon LCD self-association.</jats:p>

<jats:p> Precise <jats:italic>Hox</jats:italic> gene expression is crucial for embryonic patterning. Intra- <jats:italic>Hox</jats:italic> transcription factor binding and distal enhancer elements have emerged as the major regulatory modules controlling <jats:italic>Hox</jats:italic> gene expression. However, quantifying their relative contributions has remained elusive. Here, we introduce “synthetic regulatory reconstitution,” a conceptual framework for studying gene regulation, and apply it to the <jats:italic>HoxA</jats:italic> cluster. We synthesized and delivered variant rat <jats:italic>HoxA</jats:italic> clusters (130 to 170 kilobases) to an ectopic location in the mouse genome. We found that a minimal <jats:italic>HoxA</jats:italic> cluster recapitulated correct patterns of chromatin remodeling and transcription in response to patterning signals, whereas the addition of distal enhancers was needed for full transcriptional output. Synthetic regulatory reconstitution could provide a generalizable strategy for deciphering the regulatory logic of gene expression in complex genomes. </jats:p>

<jats:p>The past 2 years have been a strange and difficult time for the world. The COVID-19 pandemic has brought illness, hospitalization, and death near to many people. In the United States, people are divided not only on what they should do but also on what constitutes the facts. Many are seemingly in an alternative world, driven by disinformation, conspiracy theories, and anti-science beliefs. How can health and medical leaders do their jobs while trying to cope with a polarized public? They must be more effective in explaining and persuading the public on matters of science and health. This will require better clarification of two things to the public—the roles of science and politics in public policy decisions, and the means by which scientific truth is established and updated.</jats:p>

<jats:p>Horizon Europe grants held hostage over Brexit dispute</jats:p>

<jats:p>Authors and editors ignored warnings about citing noted fraudster, exposing a problem in scholarly publishing</jats:p>

<jats:p>Deep carbon exhumed by volcanic rift between Greenland and Europe implicated in 56-million-year-old hothouse</jats:p>

<jats:p>Plans to dam the upper Neretva River draw concern</jats:p>

<jats:p>Patient groups, hospitals urge continued support for the Undiagnosed Diseases Network</jats:p>

<jats:p>Bacteria and fungi behind cheese, soy, and more share genomic traits wth domesticated plants and animals</jats:p>